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| Term | beta galactosidase complementation | ID (Ontology) | MI:0010 (Molecular Interactions) | |||||
| Definition | Beta-galactosidase activity can be used to monitor the interaction of chimeric proteins. Pairs of inactive beta gal deletion mutants are capable of complementing to restore activity when fused to interacting protein partners. Critical to the success of this system is the choice of two poorly complementing mutant moieties, since strongly complementing mutants spontaneously assemble and produce functional beta-gal activity detectable in absence of any fused protein fragment.[ PubMed:12042868 PubMed:9237989 ] | |||||||
| Also Known As | "beta galactosidase" | |||||||
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experimental interaction detection |__protein complementation assay |__beta galactosidase complementation 1 rec. |
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| Is a | protein complementation assay | ||
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