Allele Dmel\ci^W

General Information
Symbol	Dmel\ci^W	Species	D. melanogaster
Name	of Wallace	FlyBase ID	FBal0001653
Feature type	allele	Associated gene	Dmel\ci

Allele class	gain of function allele
Mutagen	spontaneous
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	gain of function allele (Dominguez, 1999)
Mutagen	spontaneous (Slusarski et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Uncharacterised alterations located 5' to the transcription unit and 10kb 3' to the transcription unit. (Slusarski et al., 1995) Single 6.4kb insertion in the PstI HaeIII fragment. (Locke and Tartof, 1994) 4.0 kb insert, Different from the insert in ci³⁶. ci¹, ci³⁶, ci⁵⁷, ci^D and ci^W mutant in the same 5.8 kb Bgl fragment. This fragment hybridizes to a 2.0 kb RNA that shows peak concentrations in late pupae.
Caused by insertion	?{}ci^W (Lindsley and Zimm, 1992)
Cytology
Phenotypic Data
Phenotypic Class
	visible (Sanchez-Herrero et al., 1996) visible \| dominant (Campbell and Tomlinson, 2000, Diaz-Benjumea and Garcia-Bellido, 1990, Svendsen et al., 2000) visible \| dominant \| cold sensitive (Locke and Hanna, 1996)
Phenotype Manifest In
	leg (with ci^D) (Sanchez-Herrero et al., 1996) ommatidial precursor cluster (Dominguez, 1999) wing (Dominguez et al., 1996) wing \| anterior compartment (Sanchez-Herrero et al., 1996) wing vein (Locke and Hanna, 1996) wing vein L4 (Campbell and Tomlinson, 2000, Svendsen et al., 2000)
Detailed Description
	Statement Reference Heterozygotes show variable loss of vein L4 tissue. (Campbell and Tomlinson, 2000) Heterozygotes show wing vein L4 defects. (Svendsen et al., 2000) When analysed in large clones in the developing eye the packing in the ommatidial clusters is disrupted. (Dominguez, 1999) Wings from homozygotes have, at low penetrance, a mirror symmetrical outgrowth composed of anterior wing material, resembling duplications caused by ectopic dpp expression. (Dominguez et al., 1996) Homozygotes occasionally show overgrowth in the anterior compartment of the wing. ci^D/ci^W flies have abnormal legs. (Sanchez-Herrero et al., 1996) Homozygous viable. (Locke and Tartof, 1994) Lack all longitudinal and transverse veins and affect morphogenesis of legs and eyes. (Diaz-Benjumea and Garcia-Bellido, 1990) Homozygote is extreme ci type. Wings sometimes almost twice normal width, arclike, and virtually lack veins. Often present is a well-organized pattern of venation in which the posterior crossvein flows smoothly into L5. Legs lumpy, sex combs larger than normal, antennae enlarged, eyes smaller and extra bristles present. Heterozygote shows gap in L4 in 80% of flies. ci^W enhanced by H, en, and Cy (House, 1953c; House 1959) and by Tp(4;Y) (Benner, 1972). Temperature effect described by House (1955b). RK2.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ci^W has visible \| dominant phenotype, enhanceable by cg[+]/cg³ (Svendsen et al., 2000) ci^W has visible \| dominant phenotype, enhanceable by cg[+]/cg⁰⁷⁶⁵⁹ (Svendsen et al., 2000, Campbell and Tomlinson, 2000)
Phenotype Manifest In
Enhanced by
Statement Reference ci^W has phenotype, enhanceable by cg¹ (House, 1953) ci^W has wing phenotype, enhanceable by en^Enci (Locke and Hanna, 1996) ci^W has wing phenotype, enhanceable by ph-p⁴¹⁰ (Randsholt et al., 2000) ci^W has wing phenotype, enhanceable by Psc¹ (Randsholt et al., 2000) ci^W has wing vein L4 phenotype, enhanceable by cg[+]/cg³ (Svendsen et al., 2000) ci^W has wing vein L4 phenotype, enhanceable by cg[+]/cg⁰⁷⁶⁵⁹ (Svendsen et al., 2000, Campbell and Tomlinson, 2000)
Suppressed by
Statement Reference ci^W has wing phenotype, suppressible by Dp(1;2;Y)w⁺ (Randsholt et al., 2000)
NOT suppressed by
Statement Reference ci^W/ci^D has leg phenotype, non-suppressible by fu^J (Sanchez-Herrero et al., 1996) ci^W/ci^D has wing phenotype, non-suppressible by fu^J (Sanchez-Herrero et al., 1996) ci^W has phenotype, non-suppressible by su(Hw)² (Lindsley and Grell, 1968)
Additional Comments
Genetic Interactions
Statement Reference The ci^W/+ wing vein L4 phenotype is enhanced by one copy of cg⁰⁷⁶⁵⁹. (Campbell and Tomlinson, 2000) The wing vein L4 defects observed in ci^W/+ heterozygotes are enhanced by one copy of cg⁰⁷⁶⁵⁹ or cg³. (Svendsen et al., 2000) Wing phenotype is enhanced by en^Enci at 18^oC. (Locke and Hanna, 1996) The wing and leg phenotypes of ci^D/ci^W flies are not altered by fu^J. (Sanchez-Herrero et al., 1996) When doubly heterozygous with en loss of function alleles, the dominant ci phenotype is enhanced. Three doses of en⁺ suppress expression of the ci phenotype in ci^W heterozygotes. (Locke and Hanna, 1995)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	ci^Ce-r1 (Kronhamn and Rasmuson-Lestander, 1999)
Fails to complement	ci¹ (Kronhamn and Rasmuson-Lestander, 1999) ci³⁶ (Kronhamn and Rasmuson-Lestander, 1999) ci⁵⁷ (Kronhamn and Rasmuson-Lestander, 1999) ci^Ce-2 (Kronhamn and Rasmuson-Lestander, 1999) ci^D (Kronhamn and Rasmuson-Lestander, 1999)
Comments
Stocks ( 2 )
Bloomington	646 ci^W
Kyoto	106024 ci^W
Notes on Origin
Discoverer	Wallace, 20th April 1936.

Comments
	Weak gain of function allele. (Dominguez, 1999) Strength of ci alleles showing a ci phenotype in combination with en^Enci can be ordered: ci¹ < ci³⁶ < ci⁵⁷ << ci^W. (Locke and Hanna, 1996)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	ci^W ci^w (Pilgram et al., 2011) It
Name Synonym	Interrupted of Wallace
Secondary FlyBase IDs

References ( 18 )
Research paper	Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Campbell and Tomlinson, 2000, Development 127(19): 4095--4103 Transcriptional regulation of the Hedgehog effector CI by the zinc-finger gene combgap. [FBrf0129753] Randsholt et al., 2000, Mech. Dev. 95(1-2): 89--99 polyhomeotic controls engrailed expression and the hedgehog signaling pathway in imaginal discs. [FBrf0130036] Svendsen et al., 2000, Development 127(19): 4083--4093 The combgap locus encodes a zinc-finger protein that regulates cubitus interruptus during limb development in Drosophila melanogaster. [FBrf0130102] Dominguez, 1999, Development 126(11): 2345--2353 Dual role for Hedgehog in the regulation of the proneural gene atonal during ommatidia development. [FBrf0108513] Kronhamn and Rasmuson-Lestander, 1999, Genome 42(6): 1144--1149 Genetic organization of the ci-M-pan region on chromosome IV in Drosophila melanogaster. [FBrf0125149] Alves et al., 1998, Mech. Dev. 78(1,2): 17--31 Modulation of Hedgehog target gene expression by the Fused serine-threonine kinase in wing imaginal discs. [FBrf0106032] Dominguez et al., 1996, Science 272(5268): 1621--1625 Sending and receiving the hedgehog signal: Control by the Drosophila Gli protein cubitus interruptus. [FBrf0088080] Locke and Hanna, 1996, Dev. Genet. 19(4): 340--349 engrailed gene dosage determines whether certain recessive cubitus interruptus alleles exhibit dominance of the adult wing phenotype in Drosophila. [FBrf0092613] Sanchez-Herrero et al., 1996, Mech. Dev. 55(2): 159--170 The fu gene discriminates between pathways to control dpp expression in Drosophila imaginal discs. [FBrf0087686] Slusarski et al., 1995, Genetics 139(1): 229--240 Mutations that alter the timing and pattern of cubitus interruptus gene expression in Drosophila melanogaster. [FBrf0079420] Locke and Tartof, 1994, Mol. Gen. Genet. 243(2): 234--243 Molecular analysis of cubitus interruptus (ci) mutations suggests an explanation for the unusual ci position effects. [FBrf0073755] Diaz-Benjumea and Garcia-Bellido, 1990, Rouxs Arch. Dev. Biol. 198(6): 336--354 Genetic analysis of the wing vein pattern of Drosophila. [FBrf0052918]
Review	Aza-Blanc and Kornberg, 1999, Trends Genet. 15(11): 458--462 Ci: a complex transducer of the hedgehog signal. [FBrf0122953]
Abstract	Locke and Hanna, 1995, A. Dros. Res. Conf. 36: 261A engrailed mutants permit pseudo-dominant expression of recessive cubitus interruptus alleles in the adult wing of Drosophila. [FBrf0078924] House, 1953, Genetics 38: 669--670 Some observations on the interaction of comb-gap with Hairless, engrailed and alleles at the cubitus interruptus locus in Drosophila melanogaster. [FBrf0009163]
Book	Lindsley and Zimm, 1992, The Genome of Drosophila melanogaster. The Genome of Drosophila melanogaster. [FBrf0066905] Lindsley and Grell, 1968, Publs Carnegie Instn 627: 469pp Genetic variations of Drosophila melanogaster. [FBrf0020044]

Allele Dmel\ciW

Allele Dmel\ci^W