FlyBase Allele Report: Dmel\br[npr-6]

General Information

Symbol

Dmel\br^npr-6

Species

D. melanogaster

Name

FlyBase ID

FBal0013160

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

Also Known As

npr⁶, l(1)t435, npr1⁶, l(1)t⁴³⁵, ecs^lt435

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(D'Avino et al., 1995, Gonzy-Treboul et al., 1995)

Mutagen

ethyl methanesulfonate

(Belyaeva et al., 1982, Belyaeva et al., 1981)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with br^rbp-1)

(Tzolovsky et al., 1999)

female sterile (with br^rbp-2)

(Tzolovsky et al., 1999)

lethal

(Demakova et al., 1988)

lethal (with br^rbp-2)

(Belyaeva et al., 1980)

lethal (with br^rbp-5)

(Belyaeva et al., 1980)

lethal (with Df(1)br²⁵)

(Belyaeva et al., 1980)

lethal | female (with br⁶)

(Tzolovsky et al., 1999)

lethal - all die before end of larval stage

(Belyaeva et al., 1981)

lethal - all die before end of larval stage (with br⁵)

(Tzolovsky et al., 1999)

lethal - all die before end of larval stage (with Df(1)P154)

(Belyaeva et al., 1987)

lethal - all die before end of larval stage | recessive

(D'Avino et al., 1995, Emery et al., 1994, Belyaeva et al., 1987, Belyaeva et al., 1982)

lethal - all die before end of pupal stage (with br^2Bc-2)

(Tzolovsky et al., 1999)

partially lethal (with Df(1)br²⁵)

(Belyaeva et al., 1980)

some die during larval stage

(Belyaeva et al., 1981)

some die during larval stage (with br⁵)

(Tzolovsky et al., 1999)

some die during larval stage (with Df(1)P154)

(Belyaeva et al., 1987)

some die during larval stage | recessive

(D'Avino et al., 1995, Emery et al., 1994, Belyaeva et al., 1987, Belyaeva et al., 1982)

some die during pupal stage (with br^2Bc-2)

(Tzolovsky et al., 1999)

visible (with T(1;2)dor^var7)

(Demakova et al., 1988)

Phenotype Manifest In

dorsal appendage (with br¹)

(Tzolovsky et al., 1999, Deng and Bownes, 1997)

dorsal appendage (with br^rbp-2)

(Tzolovsky et al., 1999)

maxillary palp bristle (with br^rbp-2)

(Belyaeva et al., 1980)

maxillary palp bristle (with br^rbp-5)

(Belyaeva et al., 1980)

ovary (with br^rbp-2)

(Tzolovsky et al., 1999)

wing

(Demakova et al., 1988)

wing (with Df(1)br²⁵)

(Belyaeva et al., 1980)

wing (with T(1;2)dor^var7)

(Demakova et al., 1988)

Detailed Description

Statement

Reference

br^rbp-1/br^npr-6 and produce very few offspring. br^rbp-2/br^npr-6 females live for two days before dying having laid no eggs. Their ovaries are not completely developed, and the few late stage oocytes that were formed had no appendages. The br^2Bc-2/br^npr-6 heteroallelic combination is lethal. The offspring die as pupae. The br⁵/br^npr-6 heteroallelic combination is third larval instar lethal. The br⁶/br^npr-6 heteroallelic combination produces no females. The br¹/br^npr-6 mothers produce eggs that have reduced dorsal appendages.

(Tzolovsky et al., 1999)

Female progeny br^npr-6/br¹ survive until adulthood and produce eggs with reduced dorsal appendages.

(Deng and Bownes, 1997)

Homozygotes die as late third instar larvae.

(D'Avino et al., 1995)

Lethality acts in the late third instar.

(Emery et al., 1994)

Surviving br^npr-6/T(1;2)dor^var7 females may have wing abnormalities.

(Demakova et al., 1988)

Df(1)P154/br^npr-6 larvae survive 2-3 days longer than br^npr-6 homozygotes. The early ecdysone puff at region 2B3-2B5 is active and does not regress until larval death.

(Belyaeva et al., 1987)

Late third instar larvae die at puff stage 1.

(Belyaeva et al., 1982)

Males lacking the puff at 2B5 (Df(1)S39/Dp(1;Y)Sz280) or br^npr-6 homozygotes are very similar in development. Growth and development of larvae is delayed, body size is small and larvae live two or three days longer than wild type but die as larvae. Homozygous and hemizygous larvae die without exhibiting any sign of ecdysone-inducible puff formation; culture of salivary glands in 20-OH ecdysone produces partial development of some early, but none of late ecdysone-inducible puffs and extraneous puff appears at 75CD.

(Belyaeva et al., 1981)

br^rbp-2/br^npr-6 and br^rbp-5/br^npr-6 combination is lethal, rare adult escapers show a reduction of the bristle number on the palpus. Heterozygotes with Df(1)br²⁵ have reduced viability and br wings.

(Belyaeva et al., 1980)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

br¹

(Tzolovsky et al., 1999)

Partially complements

br⁶

(Tzolovsky et al., 1999)

br¹

(Tzolovsky et al., 1999)

br^rbp-2

(Tzolovsky et al., 1999)

Fails to complement

br^23RL

(Gonzy et al., 2002)

br^25.4

(Gonzy et al., 2002)

br^38JL

(Gonzy et al., 2002)

br^80BL

(Gonzy et al., 2002)

br^BC1

(Gonzy et al., 2002)

br^BC3

(Gonzy et al., 2002)

br⁵

(Tzolovsky et al., 1999)

br^2Bc-2

(Tzolovsky et al., 1999)

br^2Bc-1

(Tzolovsky et al., 1999)

br^rbp-1

(Tzolovsky et al., 1999)

Comments

br¹/br^npr-6 flies show reduced viability. The br⁵/br^npr-6 heteroallelic combination is lethal; The lethal period is during the 3rd instar larva. br⁶/+ x br^npr-6/+ crosses lead to transheterozygous males but no females being produced. Offspring die as pupae. br^rbp-1/+ x br^npr-6/+ crosses lead to very few transheterozygous males but no females being produced. Offspring die as pupae. br^rbp-2/br^npr-6 show reduced viability. The br^2Bc-2/br^npr-6 heteroallelic combination is lethal. The offspring die as pupae. The br^2Bc-1/br^npr-6 heteroallelic combination is lethal. The offspring die as 2nd to 3rd instar larvae.

(Tzolovsky et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36562

y¹ br^npr-6/Binsn

Notes on Origin

Discoverer

Comments

No 20-OH ecdysone puffs are seen in third instar larvae.

(Belyaeva et al., 1981)

Sgs3 RNA and Gebf-I protein are absent in br^npr-6 mutant larvae.

(Georgel et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

br^ecs-lt435

br^lt435

br^npr-6

br^t435

(Huet et al., 1996)

ecs^lt435

(Belyaeva and Zhimulev, 1991, Belyaeva et al., 1987)

l(1)t435

(Georgel et al., 1993, Mazina et al., 1991, Demakova et al., 1988, Belyaeva et al., 1982, Belyaeva et al., 1981, Belyaeva et al., 1980)

l(1)t⁴³⁵

(Meister and Richards, 1996, Georgel et al., 1991)

npr1⁶

(Mugat et al., 2000, D'Avino et al., 1995)

npr⁶

(Wu et al., 2020, Sempere et al., 2003, Gonzy et al., 2002, Sempere et al., 2002, Pecasse et al., 2000, Peronnet et al., 2000, Richards et al., 1999, Tzolovsky et al., 1999, Deng and Bownes, 1997, Dubrovsky et al., 1996, Dubrovsky et al., 1994, Georgel et al., 1993)

nprl⁸

(Pokholkova et al., 1993)

Name Synonyms

Secondary FlyBase IDs

FBal0030641
FBal0030642
FBal0030644

References (33)

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