FB2025_01 , released February 20, 2025
Allele: Dmel\par-1UAS.ΔSP.GFP
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General Information
Symbol
Dmel\par-1UAS.ΔSP.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0178516
Feature type
allele
Associated gene
Dmel\par-1
Associated Insertion(s)
Carried in Construct
P{UAS-par-1.ΔSP.GFP}
Key Links
Transgenic product class
inframe_deletion
(Vaccari et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Vaccari et al., 2005)
Mutagen
in vitro construct
(Vaccari, 2005, Vaccari et al., 2005)
Progenitor genotype
par-1UAS.GFP
(Vaccari et al., 2005)
Carried in construct
P{UAS-par-1.ΔSP.GFP}
Cytology
Description

The par-1 Spacer domain has been deleted.

(Vaccari et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
par-1
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The oocyte nucleus is mislocalised to the centre of the oocyte in 96% of egg chambers of females expressing par-1Scer\UAS.ΔSP.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

      (Tian and Deng, 2009)

      When par-1Scer\UAS.ΔSP.T:Avic\GFP is expressed in par-1W3 mutant clones, stable wild-type microtubule network is seen. When par-1Scer\UAS.ΔSP.T:Avic\GFP is expressed in par-1W3 cells (driven by Scer\GAL4αTub84B.PL) follicel cell formation is rescued, however round cells are seen that protrude from some portions of an otherwise normal cuboidal epithelium. Multi-layering of the FE is evident after stage 6, which correlates with a failure to differentiate a columnar FE over the oocyte, and in severe cases results in an egg-chamber degeneration around stages 8 and 9.

      (Vaccari et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The penetrance of the oocyte mislocalisation phenotype seen in egg chambers of females expressing par-1Scer\UAS.ΔSP.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is reduced by co-expression of tauScer\UAS.P\T.T:Avic\GFP-m6 (from 96% to 33%).

      (Tian and Deng, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      par-1UAS.ΔSP.GFP/Scer\GAL4αTub84B.PL partially rescues par-1W3

      (Vaccari et al., 2005)
      Comments

      Expression of par-1Scer\UAS.ΔSP.T:Avic\GFP in Scer\GAL4αTub84B.PL follicle epithelial (FE) cells rescues the formation of the FE, though the morphology and development of these cells is abnormal.

      (Vaccari et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      par-1Scer\UAS.ΔSP.T:Avic\GFP
      par-1UAS.ΔSP.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)