FlyBase Allele Report: Dmel\Dlish[GD7133]

General Information

Symbol

Dmel\Dlish^GD7133

Species

D. melanogaster

Name

FlyBase ID

FBal0199211

Feature type

allele

Associated gene

Dmel\Dlish

Associated Insertion(s)

Carried in Construct

P{GD7133}

Also Known As

UAS-dlish-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7133}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7133

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | adult stage, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

hypoplasia | adult stage, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

hypoplasia | adult stage, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

visible | adult stage, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing blade, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

wing hair, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

wing posterior compartment, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

Detailed Description

Statement

Reference

Expression of Dlish^GD7133 reduces the size of the posterior wing compartment (driven by Scer\GAL4^hh.PU) or the whole adult wing blade and causes hair polarity defects (driven by Scer\GAL4^nub.PU) relative to controls.

(Zhang et al., 2016)

Adults expressing CG10933^GD7133 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7133

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^hh.PU/Dlish^GD7133 is a suppressor of hyperplasia | larval stage phenotype of ft⁸

(Zhang et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^hh.PU/Dlish^GD7133 is a suppressor of wing disc | larval stage phenotype of ft⁸

(Zhang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dlish^GD7133 RNAi under the control of Scer\GAL4^hh.PU suppresses (in the posterior) the wing disc overgrowth characteristic for ft⁸ mutant larvae.

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v46726

w¹¹¹⁸; P{GD7133}v46726

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7133

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG10933^GD7133

Dlish^GD7133

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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