FlyBase Allele Report: Dmel\kek5[fe148]

General Information

Symbol

Dmel\kek5^fe148

Species

D. melanogaster

Name

FlyBase ID

FBal0241007

Feature type

allele

Associated gene

Dmel\kek5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

FLPase

Nature of the Allele

Allele class

loss of function allele

(Evans et al., 2009)

Mutagen

FLPase

(Evans et al., 2009)

Progenitor genotype

P{GT1}kek5^BG02535

(Evans et al., 2009)

Caused by aberration

Df(1)fe148

Cytology

Description

A 48 kb deletion, lacking the coding exons of kek5 in addition to removing the final exon of CG32533 (ending 9 bp upstream of the start of transcription of CG12200, located within the last intron of CG32533).

(Evans et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Evans et al., 2009)

short lived | recessive

(Evans et al., 2009)

visible (with Df(1)Exel7468)

(Evans et al., 2009)

visible (with Df(1)JA27)

(Evans et al., 2009)

visible (with kek5^Δ1)

(Evans et al., 2009)

visible | recessive

(Evans et al., 2009)

Phenotype Manifest In

crossvein

(Evans et al., 2009)

crossvein (with Df(1)Exel7468)

(Evans et al., 2009)

crossvein (with Df(1)JA27)

(Evans et al., 2009)

crossvein (with kek5^Δ1)

(Evans et al., 2009)

wing blade

(Evans et al., 2009)

wing blade (with Df(1)Exel7468)

(Evans et al., 2009)

wing blade (with Df(1)JA27)

(Evans et al., 2009)

Detailed Description

Statement

Reference

Homozygous kek5^fe148 mutants produce fertile adults. However, the mutant imagos exhibit decreased viability.

kek5^fe148 and kek5^fe148/kek5^Δ1 mutant wings exhibit defects in crossvein patterning with a penetrance of about 25-30%. The frequency of crossvein defects is enhanced by hemizygosity over Df(1)Exel7468 or Df(1)JA27. kek5^fe148 mutant crossvein defects include truncated or missing posterior crossvein, twigging or forking of the crossvein, ectopic posterior crossvein material, meandering posterior crossvein and ectopic crossveins around the anterior crossvein. The anterior crossvein is rarely deleted or truncated in kek5^Δ1 mutants; more often partial or full-length ectopic crossveins appear adjacent to the anterior crossvein.

kek5^fe148 mutant wings display blisters at low frequency (less than 5%) when homo- or hemizygous, or when either placed over deficiencies for the region Df(1)Exel7468 or Df(1)JA27.

(Evans et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

kek5^fe148/kek5^fe148 is an enhancer of visible phenotype of Scer\GAL4^ptc-559.1, sog^UAS.cYa

(Evans et al., 2009)

Suppressor of

Statement

Reference

kek5^fe148/kek5^fe148 is a suppressor of visible phenotype of Scer\GAL4^ap-md544, tkv^{1AΔGS.UAS.Tag:HA}

(Evans et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

kek5^fe148/kek5^fe148 is an enhancer of crossvein phenotype of Scer\GAL4^ptc-559.1, sog^UAS.cYa

(Evans et al., 2009)

Suppressor of

Statement

Reference

kek5^fe148/kek5^fe148 is a suppressor of wing vein L4 phenotype of Scer\GAL4^ap-md544, tkv^{1AΔGS.UAS.Tag:HA}

(Evans et al., 2009)

kek5^fe148/kek5^fe148 is a suppressor of wing cell phenotype of Scer\GAL4^ptc-559.1, sog^{supersog1.UAS}

(Evans et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The ectopic crossvein phenotype in wings expressing sog^Scer\UAS.cYa driven by Scer\GAL4^ptc-559.1 is enhanced by kek5^fe148. A full-length ectopic crossvein appears between L4 and L5 in 33% of wings expressing sog^Scer\UAS.cYa driven by Scer\GAL4^ptc-559.1 in a homozygous kek5^fe148 background.

A homozygous kek5^fe148 genetic background suppresses the wing vein L4 phenotypes engendered by Scer\GAL4^ap-md544 driven expression of tkv^{1AΔGS.Scer\UAS.T:Ivir\HA1}.

The loss of intervein area between L4 and L5 resulting from expression of sog^{supersog1.Scer\UAS} with Scer\GAL4^ptc-559.1 in the wing is suppressed in a homozygous kek5^fe148 mutant background.

(Evans et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

kek5^fe148

(Evans et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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