Allele Dmel\Caf1-180^Scer\UAS.cSa

General Information
Symbol	Dmel\Caf1-180^Scer\UAS.cSa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Song	FlyBase ID	FBal0267423
Feature type	allele	Associated gene	Dmel\Caf1-180

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Song et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a full length Caf1-180 cDNA. (Song et al., 2007)
Carried in construct	P{UAS-Caf1-180.S} (Song et al., 2007, Wen et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^ey.PH (Song et al., 2007) size defective \| adult stage, with Scer\GAL4^ey.PH (Wen et al., 2012) visible, with Scer\GAL4^ey.PH (Song et al., 2007) visible \| adult stage, with Scer\GAL4^ey.PH (Wen et al., 2012)
Phenotype Manifest In
	eye, with Scer\GAL4^ey.PH (Song et al., 2007, Wen et al., 2012)
Detailed Description
	Statement Reference Flies expressing Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ey.PH] have small eyes compared to wild type. The phenotype is stronger with two copies of the transgene compared with one copy. (Wen et al., 2012) Eye-specific expression of Caf1-180[Scer\UAS.cSa] driven by Scer\GAL4[ey.PH] results in a small-eye phenotype. (Song et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has visible \| adult stage phenotype, non-enhanceable by Caf1^Scer\UAS.cWa, Scer\GAL4^ey.PH (Wen et al., 2012) Caf1-180^Scer\UAS.cSa has size defective \| adult stage phenotype, non-enhanceable by Caf1^Scer\UAS.cWa/Scer\GAL4^ey.PH (Wen et al., 2012)
Suppressed by
Statement Reference Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has increased cell death phenotype, suppressible by asf1[+]/asf1¹ (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has increased cell death phenotype, suppressible by asf1^Scer\UAS.cMa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has increased cell death phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has increased cell death phenotype, suppressible by Blm^Scer\UAS.cSa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has increased cell death phenotype, suppressible by mus309[+]/Blm^D2 (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has visible phenotype, suppressible by asf1[+]/asf1¹ (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has visible phenotype, suppressible by asf1^Scer\UAS.cMa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has visible phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has visible phenotype, suppressible by Blm^Scer\UAS.cSa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has visible phenotype, suppressible by mus309[+]/Blm^D2 (Song et al., 2007)
Phenotype Manifest In
Suppressed by
Statement Reference Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has eye phenotype, suppressible by asf1[+]/asf1¹ (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has eye phenotype, suppressible by asf1^Scer\UAS.cMa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has eye phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has eye phenotype, suppressible by Blm^Scer\UAS.cSa, Scer\GAL4^ey.PH (Song et al., 2007) Caf1-180^Scer\UAS.cSa, Scer\GAL4^ey.PH has eye phenotype, suppressible by mus309[+]/Blm^D2 (Song et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Caf1[Scer\UAS.cWa] does not enhance the small eye phenotype seen when Caf1-180[Scer\UAS.cSa] is expressed under the control of Scer\GAL4[ey.PH]. (Wen et al., 2012) The small-eye phenotype is suppressed in flies co-expressing Blm[Scer\UAS.cSa] with Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ey.PH]. The small-eye phenotype is suppressed in flies co-expressing asf1[Scer\UAS.cMa] with Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ey.PH]. The small-eye phenotype of flies expressing Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ey.PH] is suppressed by heterozygous Blm[D2]. The small-eye phenotype of flies expressing Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ey.PH] is suppressed by heterozygous asf1[1]. (Song et al., 2007)
Xenogenetic Interactions
Statement Reference The small-eye phenotype is suppressed in flies co-expressing BacA\p35[Scer\UAS.cHa] with Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ey.PH]. (Song et al., 2007)
Complementation & Rescue Data
Rescues	Caf1-180^Scer\UAS.cSa rescues Caf1-180²⁷⁰ (Song et al., 2007)
Comments	Ubiquitous expression of Caf1-180[Scer\UAS.cSa] under the control of Scer\GAL4[ptc-559.1] completely rescues the mutant phenotypes associated with Caf1-180[270]. (Song et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Caf1-180^Scer\UAS.cSa Caf1^Scer\UAS.cSa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Song
Secondary FlyBase IDs

References ( 2 )
Research paper	Wen et al., 2012, Dev. Dyn. 241(3): 455--464 The biological function of the WD40 repeat-containing protein p55/Caf1 in Drosophila. [FBrf0217472] Song et al., 2007, Dev. Biol. 311(1): 213--222 CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. [FBrf0200457]

Allele Dmel\Caf1-180Scer\UAS.cSa

Allele Dmel\Caf1-180^Scer\UAS.cSa