FlyBase Allele Report: Dmel\Pi3K68D[KD.UAS.Tag:MYC]

General Information

Symbol

Dmel\Pi3K68D^{KD.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0282701

Feature type

allele

Associated gene

Dmel\Pi3K68D

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K68D.KD}

Key Links

Transgenic product class

dominant_negative_variant

(MacDougall et al., 2004)

missense_variant

(MacDougall et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(MacDougall et al., 2004)

missense_variant

(MacDougall et al., 2004)

Mutagen

in vitro construct

(MacDougall et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Pi3K68D.KD}

Cytology

Description

UASt regulatory sequences drive expression of a kinase dead form of Pi3K68D (contains the amino acid replacement D1457A) which is tagged at the N-terminal end with Tag:MYC.

(MacDougall et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pi3K68D

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^69B

(MacDougall et al., 2004)

visible, with Scer\GAL4^ptc-559.1

(MacDougall et al., 2004)

Phenotype Manifest In

campaniform sensillum | ectopic, with Scer\GAL4^69B

(MacDougall et al., 2004)

chaeta | increased number, with Scer\GAL4^69B

(MacDougall et al., 2004)

scutellar bristle, with Scer\GAL4^ptc-559.1

(Powis and Macdougall, 2011, MacDougall et al., 2004)

wing margin, with Scer\GAL4^69B

(MacDougall et al., 2004)

wing vein | ectopic, with Scer\GAL4^69B

(MacDougall et al., 2004)

Detailed Description

Statement

Reference

Expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} in the scutellum, under the control of Scer\GAL4^ptc-559.1, results in an increase in scutellar bristles.

(Powis and Macdougall, 2011)

Expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 results in an increase in the average number of scutellar bristles compared to wild type.

Expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B at 25[o]C results in very minor amounts of additional wing vein material in most flies, while a small proportion show more severe defects, with extensive ectopic wing venation and loss of wing margin. The number of campaniform sensilla on the wing are increased and there are supernumerary bristles on the thorax.

(MacDougall et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has visible phenotype, enhanceable by Egfr[+]/Egfr^E1

(MacDougall et al., 2004)

NOT Enhanced by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has visible phenotype, non-enhanceable by drk[+]/drk¹⁰⁶²⁶

(MacDougall et al., 2004)

Suppressed by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has visible phenotype, suppressible by drk[+]/drk^e0A

(MacDougall et al., 2004)

NOT suppressed by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has visible phenotype, non-suppressible by drk[+]/drk¹⁰⁶²⁶

(MacDougall et al., 2004)

Enhancer of

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}/Scer\GAL4^69B is an enhancer of visible | dominant phenotype of N^264-39

(MacDougall et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has wing vein | ectopic phenotype, enhanceable by Egfr[+]/Egfr^E1

(MacDougall et al., 2004)

NOT Enhanced by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has wing vein | ectopic phenotype, non-enhanceable by drk[+]/drk¹⁰⁶²⁶

(MacDougall et al., 2004)

Suppressed by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has wing vein | ectopic phenotype, suppressible by drk[+]/drk^e0A

(MacDougall et al., 2004)

NOT suppressed by

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B has wing vein | ectopic phenotype, non-suppressible by drk[+]/drk¹⁰⁶²⁶

(MacDougall et al., 2004)

Enhancer of

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}/Scer\GAL4^69B is an enhancer of wing margin phenotype of N^264-39

(MacDougall et al., 2004)

Suppressor of

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B is a suppressor of wing vein L4 phenotype of Scer\GAL4^69B, aos^UAS.cHa

(MacDougall et al., 2004)

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B is a suppressor of anterior crossvein phenotype of Scer\GAL4^69B, aos^UAS.cHa

(MacDougall et al., 2004)

Other

Statement

Reference

Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B, aos^UAS.cHa has posterior crossvein phenotype

(MacDougall et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Egfr^E1/+ enhances the formation of an ectopic crossvein in flies expressing Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B at 25[o]C.

Co-expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} suppresses the loss of wing vein L4 and the anterior crossvein caused by expression of aos^Scer\UAS.cHa under the control of Scer\GAL4^69B, but the posterior crossvein is lost in the double mutant flies.

The wing notching phenotype seen in N^264-39/+ flies is enhanced by expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B.

drk^e0A/+, but not drk¹⁰⁶²⁶/+, suppresses the ectopic wing vein phenotype caused by expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B.

(MacDougall et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC}

Pi3K68D^{KD.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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