FB2025_01 , released February 20, 2025
Allele: Dmel\Ptp61FΔ
Open Close
General Information
Symbol
Dmel\Ptp61FΔ
Species
D. melanogaster
Name
FlyBase ID
FBal0284953
Feature type
allele
Associated gene
Dmel\Ptp61F
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Buszard et al., 2013)
Mutagen
Delta2-3 transposase
(Buszard et al., 2013)
Progenitor genotype
P{RS5}Ptp61F5-HA-2445
(Buszard et al., 2013)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 2098bp deletion that extends 970bp upstream and 1128bp downstream of the original insertion site. This deletion removes the first exon of the Ptp61F m and n isoforms.

(Buszard et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:1,349,414..1,351,512 [-]
Comment:

2098 bp deletion resulting from the imprecise excision of P{RS5}Ptp61F5-HA-2445, which removes sequence extending from 970 bp upstream to 1128 bp downstream of the insertion site.

(Buszard et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA with Ras85DV12.cUa.UAS
      (Liu et al., 2022)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  cancer
      modeled by Ras85DV12.cUa.UAS
      (Liu et al., 2022)
      ameliorates  cancer
      modeled by sav3
      (Liu et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous flies are normal in size (as measured by wing centroid size).

      Homozygous females show a significant reduction in life span. Male fertility is normal, but female fecundity (number of eggs laid per fly per day) is reduced compared to wild type. The number and morphology of somatic cells surrounding the developing egg chambers appear normal in mutant ovaries. However, late stage egg chambers (particularly stage 10 or later) are missing, and there is an accumulation of germline stem cells and/or early stage egg chambers. There is an approximately 8-fold increase in the number of apoptotic pre-stage 10 egg chambers compared to wild type.

      (Buszard et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Ptp61FΔ has decreased fecundity | female phenotype, suppressible | partially by Stat92EGD4492/Scer\GAL4arm.PU

      (Buszard et al., 2013)
      NOT suppressed by
      Statement
      Reference

      Ptp61FΔ has decreased fecundity | female phenotype, non-suppressible by Pi3K92ED954A.UAS.Tag:MYC/Scer\GAL4arm.PU

      (Buszard et al., 2013)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4arm.PU fails to rescue the reduced fecundity of Ptp61FΔ females.

      Expression of Stat92EGD4492 under the control of Scer\GAL4arm.PU partially rescues the reduced fecundity of Ptp61FΔ females.

      (Buszard et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Ptp61FΔ is rescued by Ptp61FUAS.m.Tag:MYC/Scer\GAL4arm.PU/Ptp61FUAS.n.Tag:MYC

      (Buszard et al., 2013)
      Partially rescued by

      Ptp61FΔ is partially rescued by Ptp61FUAS.m.Tag:MYC/Scer\GAL4arm.PU

      (Buszard et al., 2013)

      Ptp61FΔ is partially rescued by Scer\GAL4arm.PU/Ptp61FUAS.n.Tag:MYC

      (Buszard et al., 2013)
      Not rescued by

      Ptp61FΔ is not rescued by Scer\GAL4Act.PU/Ptp61FUAS.m.Tag:MYC

      (Buszard et al., 2013)
      Comments

      Expression of Ptp61FScer\UAS.n.T:Hsap\MYC under the control of Scer\GAL4arm.PU fails to rescue the reduced lifespan of Ptp61FΔ flies. The reduced fecundity of Ptp61FΔ females is partially rescued.

      Simultaneous co-expression of Ptp61FScer\UAS.m.T:Hsap\MYC and Ptp61FScer\UAS.n.T:Hsap\MYC under the control of Scer\GAL4arm.PU rescues the reduced lifespan of Ptp61FΔ flies. The reduced fecundity of Ptp61FΔ females is also largely rescued.

      Expression of Ptp61FScer\UAS.m.T:Hsap\MYC under the control of Scer\GAL4Act.PU fails to rescue the reduced lifespan of Ptp61FΔ flies.

      Expression of Ptp61FScer\UAS.m.T:Hsap\MYC under the control of Scer\GAL4arm.PU partially rescues the reduced fecundity of Ptp61FΔ females.

      (Buszard et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PTP61FΔ
      (Buszard et al., 2013)
      Ptp61FΔ
      (Liu et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)