FlyBase Allele Report: Dmel\TBPH[UAS.cDa]

General Information

Symbol

Dmel\TBPH^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Diaper

FlyBase ID

FBal0287629

Feature type

allele

Associated gene

Dmel\TBPH

Associated Insertion(s)

Carried in Construct

P{UAS-TBPH.D}

Also Known As

UAS-TBPH

Key Links

Transgenic product class

cDNA

(Diaper et al., 2013)

wild_type

(Diaper et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Diaper et al., 2013)

wild_type

(Diaper et al., 2013)

Mutagen

in vitro construct

(Diaper et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-TBPH.D}

Cytology

Description

UASt regulatory sequences drive expression of a TBPH cDNA (GH09868).

(Diaper et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

TBPH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Diaper et al., 2013)

model of amyotrophic lateral sclerosis

CEA

(Diaper et al., 2013)

model of frontotemporal dementia

CEA

(Diaper et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal jump response | adult stage, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

abnormal locomotor behavior | second instar larval stage, with Scer\GAL4^Mef2.PR

(Diaper et al., 2013)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^VGlut1-OK371

(Sreedharan et al., 2015)

abnormal neurophysiology | adult stage, with Scer\GAL4^GMR.PU

(Diaper et al., 2013)

decreased rate of adult locomotory behavior, with Scer\GAL4^unspecified

(Barnard et al., 2024)

lethal - all die before end of larval stage, with Scer\GAL4^Mef2.PR

(Diaper et al., 2013)

lethal - all die during larval stage, with Scer\GAL4^repo

(Diaper et al., 2013)

majority die during second instar larval stage, with Scer\GAL4^Mef2.PR

(Diaper et al., 2013)

partially lethal - majority die, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

short lived, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

short lived, with Scer\GAL4^unspecified

(Barnard et al., 2024)

some die during pupal stage, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

some die during third instar larval stage, with Scer\GAL4^elav.PU

(Diaper et al., 2013)

Phenotype Manifest In

motor neuron | somatic clone, with Scer\GAL4^VGlut1-OK371

(Sreedharan et al., 2015)

Detailed Description

Statement

Reference

Motor neuron clones expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^VGlut-OK371 exhibit clear motor axon and NMJ degeneration.

(Sreedharan et al., 2015)

Expression of TBPH^Scer\UAS.cDa under the control of Scer\GAL4^Mef2.PR results in premature lethality, with the majority of animals dying during the second larval instar and none developing later than the third larval instar. Mutant second instar larvae show significantly impaired body wall peristalsis compared to controls. Sarcoplasmic aggregates containing TBPH protein are seen in the body wall muscles of mutant second instar larvae.

Animals expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^repo die during larval stages.

(Diaper et al., 2013)

Only around 10% of flies expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU eclose as adults. The majority of flies die during late larval/pupal stages. The remaining flies have shortened lifespan, dying at around day 35 in comparison with wild type controls that have a lifespan of ~80 days. Third instar larvae exhibit impaired peristalsis and defective locomotion and adults have non-existent or severely impaired innate escape and climbing behaviours. Flies expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^EB1 show a reduction in walking activity over time, activity and total distance travelled compared with controls. However the mean walking velocity is comparable with controls. Severe gait abnormalities are seen when TBPH^Scer\UAS.cDa is expressed under the control of Scer\GAL4^elav.PU.

Flies expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU show no difference in either the amplitude or frequency of miniature excitatory junction potentials (mEJPs). Low-frequency stimulation showed no difference in evoked excitatory junction potential, and quantal content is similar to controls. No differences are seen following high-frequency stimulation.

The electroretinograms from 1 and 5 day old flies expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PU in response to blue light pulses show a reduction in peak-peak amplitude compared to controls. No off/on transients are visible.

Expression of TBPH^Scer\UAS.cDa in the upper motor neurons under the control of Scer\GAL4^EB1 does not significantly alter lifespan.

Flies expressing TBPH^Scer\UAS.cDa under the control of Scer\GAL4^EB1 show axonal extensions and synaptic arborisations of ellipsoid body ring neurons. The phenotype worsens over time.

(Diaper et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^unspecified, TBPH^UAS.cDa has decreased rate of adult locomotory behavior phenotype, non-enhanceable by Hsap\ANXA11^{G175R.UAS.Tag:MYC}, Scer\GAL4^unspecified

(Barnard et al., 2024)

Scer\GAL4^unspecified, TBPH^UAS.cDa has short lived phenotype, non-enhanceable by Hsap\ANXA11^{G175R.UAS.Tag:MYC}, Scer\GAL4^unspecified