FlyBase Allele Report: Mmus\Prkaca[act.4xUAS]

General Information

Symbol

Mmus\Prkaca^act.4xUAS

Species

M. musculus

Name

FlyBase ID

FBal0294080

Feature type

allele

Associated gene

Mmus\Prkaca

Associated Insertion(s)

Carried in Construct

P{4xUAS-PKA.act}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Walker et al., 2013)

Progenitor genotype

Carried in construct

P{4xUAS-PKA.act}

Cytology

Description

UAS regulatory sequences containing 4 GAL4 protein-binding sites drive expression of a constitutively active form of Mmus\Prkaca.

(Walker et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mmus\Prkaca

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PU

(Walker et al., 2013)

viable, with Scer\GAL4^elav.PU

(Walker et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Aug21/Mmus\Prkaca^act.4xUAS is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Akh.PW/Mmus\Prkaca^act.4xUAS is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Mmus\Prkaca^act.4xUAS is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Mmus\Prkaca^act.4xUAS/Scer\GAL4^C23 is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Other

Statement

Reference

Mmus\Prkaca^act.4xUAS, Nf1^E2, Scer\GAL4^C23 has lethal - all die during pupal stage phenotype

(Walker et al., 2013)

Mmus\Prkaca^act.4xUAS, Nf1^E2, Scer\GAL4^Act5C.PU has lethal phenotype

(Walker et al., 2013)

Mmus\Prkaca^act.4xUAS, Nf1^E2, Scer\GAL4^Feb36 has lethal phenotype

(Walker et al., 2013)

Mmus\Prkaca^act.4xUAS, Nf1^E2, Scer\GAL4^Ras64B.PW has lethal phenotype

(Walker et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Pkaca^{act.4xScer\UAS} under the control of either Scer\GAL4^Aug21 or Scer\GAL4^Akh.PW suppresses the reduced pupal length phenotype seen in Nf1^E2 animals, while expression under the control of either Scer\GAL4^elav.PU or Scer\GAL4^C23 does not significantly suppress the phenotype.

(Walker et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmus\Pkaca^{act.4xScer\UAS}

Mmus\Prkaca^{act.4xScer\UAS}

Mmus\Prkaca^act.4xUAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close