FB2025_01 , released February 20, 2025
Allele: Scer\GAL4Ogdh1-MI06026-TG4.1
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General Information
Symbol
Scer\GAL4Ogdh1-MI06026-TG4.1
Species
S. cerevisiae
Name
FlyBase ID
FBal0325010
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Mi{Trojan-GAL4.1}Ogdh1MI06026-TG4.1
Carried in Construct
Also Known As
dOgdh-T2A-Gal4
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Yoon et al., 2017, Gene Disruption Project members, 2016-)
Progenitor genotype
Mi{MIC}Ogdh1MI06026
(Yoon et al., 2017)
Associated Insertion(s)
Mi{Trojan-GAL4.1}Ogdh1MI06026-TG4.1
Cytology
Description

Scer\GAL4 expression is driven by the endogenous Nc73EF promoter after replacing the Mi{MIC} casette in Mi{MIC}Nc73EFMI06026 with a T2A-GAL4 cassette (includes a splice acceptor, linker, ribosomal skipping T2A peptide sequence, Scer\GAL4 coding sequence, polyadenylation signal and a splice donor).

(Yoon et al., 2017)

The RMCE cassette of the Mi{MIC}Nc73EFMI06026 insertion has been replaced with a Trojan-GAL4 cassette. The insertion is in within the coding intron of all annotated transcripts of Nc73EF and is predicted to express GAL4 in the same pattern as Nc73EF.

(Gene Disruption Project members, 2016-)
Allele components
Component
Use(s)
Inserted element
Mi{Trojan-GAL4.1}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Ogdh1A343V.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1D507V.UAS.Tag:FLAG rescues Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1P867A.UAS.Tag:FLAG rescues Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Ogdh1R261W.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Ogdh1UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1W237C.UAS.Tag:FLAG rescues Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Ogdh1UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Ogdh1UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Ogdh1UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1MI06026-TG4.1/Df(3L)ED4685

      (Lee et al., 2018)

      Ogdh1UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 rescues Ogdh1MI06026-TG4.1

      (Yoon et al., 2017)
      Fails to rescue

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1F749S.UAS.Tag:FLAG fails to rescue Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1R316G.UAS.Tag:FLAG fails to rescue Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1R688Q.UAS.Tag:FLAG fails to rescue Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Ogdh1S793L.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 fails to rescue Ogdh1gRNA.pCFD3

      (Yap et al., 2021)

      Ogdh1A343V.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1D507V.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1F749S.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1P867A.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Ogdh1R261W.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1R316G.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1R688Q.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Ogdh1S793L.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1W237C.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Scer\GAL4Ogdh1-MI06026-TG4.1/Ogdh1N324S.UAS.Tag:FLAG fails to rescue Ogdh1MI06026-TG4.1

      (Yap et al., 2021)

      Ogdh1S793L.UAS.Tag:FLAG/Scer\GAL4Ogdh1-MI06026-TG4.1 fails to rescue Ogdh1MI06026-TG4.1

      (Yoon et al., 2017)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Scer\GAL4Nc73EF-MI06026-TG4.1
      Scer\GAL4Ogdh-MI06026-TG4.1
      Scer\GAL4Ogdh1-MI06026-TG4.1
      Name Synonyms
      Secondary FlyBase IDs
        References (7)