UASt regulatory sequences drive expression of the Hsap\MRTFB open reading frame, tagged at the C-terminal end with three copies of Tag:HA. The ORF was obtained from the human ORFeome v8.1 collection ( ORF_ID:14163 ). The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
Hsap\MRTFBUAS.Tag:HA/Scer\GAL4Mrtf-T2A.A is a non-suppressor of visible | adult stage phenotype of MrtfT2A.A/MrtfΔ7
Hsap\MRTFBUAS.Tag:HA, Scer\GAL4nub.PK, bsUAS.ORF.GW.Tag:HA has visible | adult stage phenotype
Hsap\MRTFBUAS.Tag:HA/Scer\GAL4Mrtf-T2A.A is a non-suppressor of bristle of mesothoracic tergum phenotype of MrtfT2A.A/MrtfΔ7
Hsap\MRTFBUAS.Tag:HA, Scer\GAL4nub.PK, bsUAS.ORF.GW.Tag:HA has wing phenotype
Hsap\MRTFBUAS.Tag:HA, Scer\GAL4nub.PK, bsUAS.ORF.GW.Tag:HA has longitudinal vein | absent phenotype
Hsap\MRTFBUAS.Tag:HA, Scer\GAL4nub.PK, bsUAS.ORF.GW.Tag:HA has crossvein | absent phenotype