FlyBase Allele Report: Dmel\Sgsf[KO]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\Sgsf^KO

General Information

Symbol

Dmel\Sgsf^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0373203

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Li et al., 2022)

Mutagen

(Li et al., 2022)

Progenitor genotype

Cytology

Description

Two short DNA regions within the open reading frame of the Sgsf gene are deleted, leading to a frameshift mutation and early termination of translation.

(Li et al., 2022)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

3R:12,341,706..12,341,709 [+]

Comment:

First of two small deletions within the Sgsf coding region. The result of this 4bp deletion is a frameshift and translation termination after the addition of nine novel amino acids.

(Li et al., 2022)

3R:12,342,159..12,342,165 [+]

Comment:

Second of two small deletions within the Sgsf coding region. Falls after the frameshift and early translation termination.

(Loehlin et al., 2022)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage

(Li et al., 2022)

decreased body size | pupal stage

(Li et al., 2022)

decreased body weight | adult stage

(Li et al., 2022)

decreased cell size | larval stage

(Li et al., 2022)

decreased size | larval stage

(Li et al., 2022)

Phenotype Manifest In

embryonic/larval salivary gland | larval stage

(Li et al., 2022)

larval brain | larval stage

(Li et al., 2022)

larval fat cell

(Li et al., 2022)

(Li et al., 2022)

(Li et al., 2022)

Detailed Description

Statement

Reference

Sgsf^KO homozygosity leads to small larval salivary glands, wing discs, brains, and fat body cells.

(Li et al., 2022)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sgsf^KO has decreased body size | pupal stage phenotype, suppressible by Scer\GAL4^Ilp3.PB/Bhal\NaChBac^UAS.EGFP

(Li et al., 2022)

Sgsf^KO has decreased body size | pupal stage phenotype, suppressible by mTor^UAS.Tag:MYC/Scer\GAL4^Cg.PA

(Li et al., 2022)

Sgsf^KO has decreased body size | pupal stage phenotype, suppressible by Scer\GAL4^Cg.PA/InR^UAS.Exel

(Li et al., 2022)

Sgsf^KO has decreased body size | pupal stage phenotype, suppressible by Scer\GAL4^Cg.PA/S6k^TE.UAS

(Li et al., 2022)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sgsf^KO is rescued by Scer\GAL4^34B/Sgsf^UAS.Tag:HA

(Li et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sgsf^KO

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections