Reference Report

Reference
Citation	Gutjahr, T., Vanario-Alonso, C.E., Pick, L., Noll, M. (1994). Multiple regulatory elements direct the complex expression pattern of the Drosophila segmentation gene paired. Mech. Dev. 48(2): 119--128. (Export to RIS)
FlyBase ID	FBrf0078548
Publication Type	Research paper
PubMed ID	7873402
PubMed Abstract	The paired (prd) gene of Drosophila belongs to the pair-rule class of segmentation genes involved in establishing the metameric organization of the Drosophila body plan. The complex expression pattern of prd has previously been shown to depend upon a number of segmentation genes, including gap and pair-rule genes. In an attempt to characterize and analyze the regulatory regions necessary and sufficient for prd expression, we have identified an 18-kb genomic fragment, consisting of the transcribed portion of prd and 10 kb of 5'- and 5 kb of 3'-flanking region, that is able to rescue prd mutant embryos to full viability. Analysis of a series of prd-lacZ fusion constructs containing progressively reduced lengths of prd 5'-flanking sequences delimits different cis-regulatory regions. The entire 5'-flanking region directs fusion gene expression in a pattern similar, but not identical, to the endogenous prd protein pattern. This 10-kb fragment contains both activator and repressor regions that mediate the establishment of the seven-stripe prd pattern, as well as the splitting into anterior and posterior stripes for the 14-stripe expression phase. The prd intron in combination with a minimal upstream region (0.15 kb) is able to direct low levels of prd-lacZ fusion gene expression in stripes. Information for expression of the anterior dorsal spot and of the early seven-stripe pattern is located downstream of the prd coding region. We propose that regulation of prd by pair-rule and gap gene products is mediated by upstream and downstream cis-regulatory elements. Regulation during separate but overlapping phases of expression by separable regulatory regions might be a general characteristic of segmentation genes.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Aberrations (2)
	Df(2L)prd1.7 Df(2L)Prl
Alleles (18)
	Ecol\lacZ^prd.ΔC Ecol\lacZ^prd.ΔD Ecol\lacZ^prd.ΔE Ecol\lacZ^prd.ΔF Ecol\lacZ^prd.ΔG Ecol\lacZ^prd.ΔH Ecol\lacZ^prd.ΔI Ecol\lacZ^prd.ΔK Ecol\lacZ^prd.ΔL Ecol\lacZ^prd.ΔM Ecol\lacZ^prd.ΔN Ecol\lacZ^prd.ΔO Ecol\lacZ^prd.ΔP Ecol\lacZ^prd.ΔQ prd⁴ prd^+t18 ry^+t7.2 w^+mW.hs
Constructs (15)
	P{prd-SN20} P{prdΔC} P{prdΔD} P{prdΔE} P{prdΔF} P{prdΔG} P{prdΔH} P{prdΔI} P{prdΔK} P{prdΔL} P{prdΔM} P{prdΔN} P{prdΔO} P{prdΔP} P{prdΔQ}
Genes (4)
	Ecol\lacZ prd ry w
Sequence features (8)
	prd_A8_repressor prd_cc_repressor prd_deltaQ prd_P1_enhancer prd_Pstripe_enhancer prd_stripe1_enhancer prd_stripe2P_enhancer prd_zebra_enhancer
Transcripts (6)
	Ecol\lacZ^prd.ΔCRA Ecol\lacZ^prd.ΔFRA Ecol\lacZ^prd.ΔIRA Ecol\lacZ^prd.ΔLRA Ecol\lacZ^prd.ΔNRA Ecol\lacZ^prd.ΔQRA