A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}EP639EP2148 Insertion

General Information
Symbol Dmel\P{EP}EP639EP2148 Species D. melanogaster
Name FlyBase ID FBti0010884
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) EP639 Viability / fertility
Causes allele(s) EP639EP2148 Stock availability none publicly available
LINE ID EP(2)2148
Genomic Location
Chromosomal location 2R ( 53D11 ) Sequence location 2R:12,716,547..12,716,547 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0111906 FBti0041444_2 FBti0151482 FBti0104366 FBti0067754 FBti0029774 FBti0107686 FBti0028961 FBti0011055 FBti0050408 FBti0028699 FBti0034685 FBti0141143 FBti0069553 FBti0011131 FBti0010884 FBti0029056 FBti0146110 FBti0011100 FBti0028946 FBti0021276 FBti0029403 FBti0029041 FBti0106948 FBti0112392 FBti0068668 FBti0007653 FBti0054172 FBti0029492 FBti0111124 FBti0028421 FBti0068911 FBti0028981 FBti0028959 FBti0112897 FBti0028909 FBti0058972 FBti0033710 FBti0108101 FBti0034189 FBti0034184 FBti0110598 FBti0054580 FBti0125221_1 FBti0065588 FBti0010966 FBti0125221_2 FBti0108585 FBti0028998 FBti0072734 FBti0025730 FBti0036469 FBti0112889 FBti0104094 FBti0034300 FBti0103603 FBti0033874 FBti0010989 FBti0110238 FBti0028310 FBti0040060 FBti0058040 FBti0030281 FBti0068953 FBti0109894 FBti0034086 FBti0145601 FBti0111695 FBti0052202
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:12,716,547..12,716,547 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
53D11 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
53D11-53D11 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
53D11-53D12 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ254834
(FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
EP639
(Abdelilah-Seyfried et al., 2000, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
EP639EP2148
(Abdelilah-Seyfried et al., 2000, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
adult thorax
(Pena-Rangel et al., 2002)
chaeta
(Pena-Rangel et al., 2002)
scutellar bristle
(Abdelilah-Seyfried et al., 2000)
tormogen cell
(Abdelilah-Seyfried et al., 2000)
tormogen cell | ectopic
(Abdelilah-Seyfried et al., 2000)
trichogen cell
(Abdelilah-Seyfried et al., 2000)
trichogen cell | ectopic
(Abdelilah-Seyfried et al., 2000)
wing
(Pena-Rangel et al., 2002)
hide Detailed Description
Statement
Reference
pnrMD237/+ adults expressing BEST:SD02913EP2148 under the control of Scer\GAL4pnr-MD237 have a strong thoracic cleft, deformed scutellum, head defects and loss of several macrochaetae and microchaetae.
(Pena-Rangel et al., 2002)
Flies expressing BEST:SD02913EP2148 under the control of Scer\GAL4ap-md544 have a slight thoracic cleft, misaligned bristles and deformed wings.
(Pena-Rangel et al., 2002)
BEST:SD02913EP2148 expressed under the control of Scer\GAL4sca-537.4 shows a genetic interaction with HE21/+.
(Abdelilah-Seyfried et al., 2000)
Expression of BEST:SD02913EP2148 under the control of Scer\GAL4sca-537.4 results in sensory organs with two sockets and two shafts, one socket and two shafts and no socket and one shaft. Loss of scutellar macrochaetae and dot-like shaft morphology is seen.
(Abdelilah-Seyfried et al., 2000)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)2148
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 2R:12343905-12343906 confirmed by FlyBase alignment of dbGSS accession AQ254834 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EP}BEST:SD02913EP2148
(FlyBase, 1992-, FlyBase, 2005)
P{EP}EP639EP2148
 
P{EP}EP2148
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050
A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055]
Abdelilah-Seyfried et al., 2000, Genetics 155(2): 733--752
A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. [FBrf0128367]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]