A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}EY01137a Insertion

General Information
Symbol Dmel\P{EPgy2}EY01137a Species D. melanogaster
Name FlyBase ID FBti0024895
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) RapGAP1 Viability / fertility viable, fertile
Causes allele(s) Stock availability 1 publicly available
LINE ID EY01137
Genomic Location
Chromosomal location 2L ( 28B1 ) Sequence location 2L:7,576,630..7,576,630 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0019133 FBti0051993 FBti0044108 FBti0070241 FBti0071000 FBti0069431 FBti0046255 FBti0067068 FBti0044461 FBti0114998 FBti0035804 FBti0011114 FBti0113262 FBti0034265 FBti0102914 FBti0106045 FBti0033941 FBti0104766 FBti0112246 FBti0025677 FBti0110904 FBti0103081 FBti0103181 FBti0036924 FBti0105447 FBti0113041 FBti0103136 FBti0107258 FBti0110009 FBti0104362 FBti0113501 FBti0007729 FBti0010898 FBti0011291 FBti0007630 FBti0006508 FBti0077983 FBti0024895 FBti0028840 FBti0057389 FBti0036392 FBti0016428 FBti0036441 FBti0103815 FBti0069550 FBti0111281 FBti0025884 FBti0104331 FBti0026399 FBti0108340 FBti0104153 FBti0028898 FBti0109370 FBti0072748 FBti0029166 FBti0030367 FBti0068782 FBti0028285 FBti0033420 FBti0034347 FBti0034479 FBti0033613 FBti0034482 FBti0034574 FBti0108675 FBti0104816 FBti0054669 FBti0107780 FBti0111376 FBti0103063 FBti0103649 FBti0103652 FBti0055539 FBti0100782 FBti0045767 FBti0109009 FBti0146026 FBti0017191 FBti0145192
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:7,576,630..7,576,630 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
28B1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
28A6 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
BZ665544
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
RapGAP1
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY01137
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
15062
w1118; P{EPgy2}EY01137a P{EPgy2}Aats-alaEY01137b
hide Linkouts
hide Comments
Location 2L:7576629-7576630 determined by FlyBase alignment of dbGSS accession BZ665544 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EY01137
(Gene Disruption Project members, 2001-)
P{EPgy2}EY01137a
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]