miniTurboID corresponds to a promiscuous biotin ligase derived by mutation of the Escherichia coli birA gene (EcoGene:EG101230); miniTurboID contains the following mutations relative to the wild-type protein; it lacks the N-terminal domain (residues 1-63) and contains the amino acid substitutions Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V and I305V. miniTurboID can thus be used as a proximity labeling tool for proteomic analysis; it has faster labeling kinetics than BirA(R118G); 10 minute labeling using miniTurboID produces similar proteome labeling as seen for 18 hour labeling using BirA(R118G) (FBrf0240093). The exact sequence of the miniTurboID biotin ligase may vary depending on the particular transgene or modified endogenous locus being used; the coding sequence may have been codon optimized for use in different species, and amino acid residue A146 may have been deleted to suppress dimerization. For a comparison of miniTurboID with other protein proximity labeling tools, see PMID:30774936, FBrf0240093.