FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
CV Term Report
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General Information
Term biochemical ID (Ontology) MI:0401 (Molecular Interactions)
Definition The application of chemical principles and methods to biological experiments to demonstrate an interaction.[ PubMed:14755292 ]
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 Interaction Groups
 biochemical (all annotations which use CV term, excluding "NOT" statements)       1
Spanning Tree (Parents/Children)
Only view relationship:
  interaction detection method
   |__experimental interaction detection
       |__biochemical  19158 rec.
           |__affinity technology 13565 rec.
           |   |__affinity chromatography technology(+) 13295 rec.
           |   |__array technology(+) 8 rec.
           |   |__competition binding 10 rec.
           |   |__display technology(+) 19 rec.
           |   |__monoclonal antibody blockade
           |   |__protein folding/unfolding
           |   |__proximity ligation assay 143 rec.
           |   |__saturation binding
           |   |__solid phase assay(+) 264 rec.
           |   |__systematic evolution of ligands by exponential enrichment 21 rec.
           |__aggregation assay 2 rec.
           |__chromatography technology 13473 rec.
           |   |__affinity chromatography technology(+) 13295 rec.
           |   |__filter trap assay 1 rec.
           |   |__ion exchange chromatography 197 rec.
           |   |__molecular sieving 528 rec.
           |   |__reverse phase chromatography
           |__cosedimentation 4948 rec.
           |   |__cosedimentation in solution 27 rec.
           |   |__cosedimentation through density gradient 95 rec.
           |   |__field flow fractionation
           |   |__liposome binding assay
           |__cross-linking study 385 rec.
           |   |__nucleic acid uv cross-linking assay(+) 141 rec.
           |   |__protein cross-linking with a bifunctional reagent 91 rec.
           |__electrophoretic mobility-based method 513 rec.
           |   |__comigration in gel electrophoresis(+) 509 rec.
           |   |__gemma
           |__enzymatic study 560 rec.
           |   |__acetylase assay(+) 5 rec.
           |   |__adp ribosylase assay
           |   |__amidase assay
           |   |__aminoacylation assay
           |   |__ampylation assay
           |   |__carboxylation assay
           |   |__cleavage assay(+) 9 rec.
           |   |__de-ADP-ribosylation assay
           |   |__deacetylase assay(+)
           |   |__deaminase assay
           |   |__decarboxylation assay
           |   |__deformylase assay
           |   |__deglycosylase assay
           |   |__demethylase assay
           |   |__deneddylase assay
           |   |__desumoylase assay
           |   |__deubiquitinase assay 2 rec.
           |   |__diphtamidase assay
           |   |__formylase assay
           |   |__glycosylase assay
           |   |__hydroxylase assay
           |   |__isomerase assay(+)
           |   |__lipidase assay(+)
           |   |__methyltransferase assay(+) 4 rec.
           |   |__neddylase assay
           |   |__nuclease assay(+) 1 rec.
           |   |__nucleoside triphosphatase assay(+) 4 rec.
           |   |__oxidoreductase assay
           |   |__phosphatase assay(+) 6 rec.
           |   |__phospholipase assay
           |   |__phosphopantetheinylase assay
           |   |__phosphotransferase assay(+) 33 rec.
           |   |__polymerase assay(+)
           |   |__sulfurtransferase assay
           |   |__sumoylase assay
           |   |__ubiquitinase assay 1 rec.
           |   |__uridylation assay
           |__footprinting 7 rec.
           |   |__chemical footprinting(+) 1 rec.
           |   |__enzymatic footprinting(+) 2 rec.
           |   |__ultraviolet (uv) footprinting
           |__gdp/gtp exchange assay
           |__nucleotide exchange assay
           |   |__gdp/gtp exchange assay
           |__polymerization
           |__probe interaction assay 137 rec.
               |__labelling assay(+) 137 rec.
               |__monoclonal antibody blockade
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