Lethal in combination with Df(4)M101-63a, Df(4)Δ9M or Df(4)M101-62f. Inferred to overlap with: Df(4)M101-63a. Inferred to overlap with: Df(4)M101-62f. Inferred to overlap with: Df(4)Δ9M.
Df(4)Δ3M does not uncover hybrid sterility when heterozygous in males containing the D.simulans 4th chromosome introgressed into an otherwise D.melanogaster background.