Df(2R)gek^D23 has been shown to have two phenotypes in germ line clones; reduced fertility and abnormal F-actin structures. These phenotypes were previously both attributed to lack of gek function (FBrf0099374), but rescue analysis indicates that lack of enok function is fully responsible for the infertility phenotype seen in Df(2R)gek^D23 germ line clones whereas the abnormal actin structures are due to a lack of gek function.

Homozygotes tend to enter the wandering third instar larval stage at about 7 days after larval hatching (ALH) at 25^oC while their heterozygous counterparts take only 3.5 to 4.0 days. Approximately half of the animals die before pupariation formation. Those that pupate do so at around 10 days ALH. Homozygotes never eclose as adults. Homozygous mutant clones exhibit defects in the mushroom body. Mushroom bodies contain only what appears to be a γ lobe and have a reduced cell number. These phenotypes are indistinguishable from those seen in enok¹ and enok².

Germline clones produce eggs but only 5% are fertilised. <up>FlyBase curator comment: this phenotype was originally attributed to lack of gek function in this paper, but rescue analysis in FBrf0135156 indicates that it is due to the deletion of enok in Df(2R)gek^D23.</up>