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FB2026_01
,
released March 12, 2026
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
No eye phenotypes are seen in Df(1)BSC759/Df(1)ED7355 transheterozygotes.
Homozygous Df(1)BSC759 mutant females are lethal at the pupal stage and the adult escapers exhibit loss of ommatidia and disrupted rhabdomere structure in the eyes. Df(1)BSC759 mutant males show a similar eye phenotype. Disorganised ommatidia are also seen in Df(1)BSC759 mutant eye discs from third instar larvae.
Homozygous Df(1)BSC759 mutant flies results in impaired locomotive ability in both males and female two day old adult flies. The lifespan of adult flies is also shortened. Male Df(1)BSC759 mutant larva show severe reduction in locomotive activity. Examination of neuromuscular junctions in ventral longitudinal muscles 6 and 7 of segment A2 in male mutant larvae reveals a severe decrease in numbers of both big and small boutons. Df(1)BSC759 mutant males also exhibit disorganised motor neurons in neuromeres A1-A5 of the ventral nerve cord. However, nuclear staining appears similar to wild type and no apoptosis is seen.
The presence of P+PBac{XP5.WH5}BSC759 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(1)BSC759 predicted from the Release 5 genomic coordinates of the progenitor insertion sites are 14A8;14C1.