FlyBase Gene Report: Dmel\eas

General Information

Symbol

Dmel\eas

Species

D. melanogaster

Name

easily shocked

Annotation Symbol

CG3525

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000536

Gene Model Status

Current

Stock Availability

15 publicly available

Enzyme Name (EC)

choline kinase (2.7.1.32)

ethanolamine kinase (2.7.1.82)

Gene Summary

Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis. (UniProt, P54352)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

ala, Ethanolamine kinase

Key Links

Genomic Location

Cytogenetic map

14B7-14B7

Sequence location

X:16,278,328..16,285,618 [+]

Recombination map

1-53

RefSeq locus

NC_004354 REGION:16278328..16285618

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables ethanolamine kinase activity

inferred from direct assay

(Pavlidis et al., 1994)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables choline kinase activity

traceable author statement

(Engel and Wu, 1994)

enables ethanolamine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000503516

(GO Reference Genome Project, 2011-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in asymmetric neuroblast division

inferred from mutant phenotype

(Pascual et al., 2005)

involved_in brain development

inferred from mutant phenotype

(Boquet et al., 2000)

involved_in mechanosensory behavior

inferred from mutant phenotype

(Engel and Wu, 1994)

involved_in mushroom body development

inferred from mutant phenotype

(Pascual et al., 2005, Pascual and Preat, 2001)

involved_in phosphatidylethanolamine metabolic process

inferred from direct assay

(Kliman et al., 2010)

involved_in response to hypoxia

inferred from direct assay

(Whelan et al., 2010)

involved_in response to mechanical stimulus

inferred from genetic interaction with FLYBASE:kcc; FB:FBgn0261794

(Hekmat-Scafe et al., 2006)

inferred from mutant phenotype

(Engel and Wu, 1994)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in associative learning

traceable author statement

(Burrell and Sahley, 2001)

involved_in habituation

traceable author statement

(Burrell and Sahley, 2001)

involved_in long-term memory

traceable author statement

(Heisenberg, 2003)

involved_in mushroom body development

traceable author statement

(Heisenberg, 2003)

involved_in phosphatidylethanolamine biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000503516

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000503516

(GO Reference Genome Project, 2011-)

located_in cytoplasm

inferred from sequence or structural similarity with SGD:S000004123

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

UNCLASSIFIED KINASES

Protein Family (UniProt)

Belongs to the choline/ethanolamine kinase family. (P54352)

Protein Signatures (InterPro)

Protein kinase-like domain superfamily

Catalytic Activity (EC/Rhea)

ethanolamine kinase activity

ethanolamine + ATP = phosphoethanolamine + ADP + H(+) (2.7.1.82)

RHEA 13069: choline kinase activity

choline + ATP = phosphocholine + ADP + H(+) (2.7.1.32)

RHEA 12837:

Summaries

Gene Group (FlyBase)

UNCLASSIFIED KINASES -: This group comprises kinases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis.

(UniProt, P54352)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

eas: easily shocked (J.C. Hall)

Brief paralysis following exposure to mechanical shock; this phenotype suppressed by napts mutation at its permissive temperature; release of neurotransmitter at larval neuromuscular junction is apparently normal.

Summary (Interactive Fly)

ethanolamine kinase - catalyzes the first step of the synthesis of phosphatidylethanolamine, one of the three major membrane phospholipids - a behavioral mutant that belongs to a family of bang-sensitive paralytic mutants - required in mushroom body neuroblasts to allow a rapid rate of cell division at metamorphosis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eas for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P54352)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eas-RA

FBtr0074212

NM_078640

2195

495

eas-RB

FBtr0074211

NM_167490

2263

495

eas-RC

FBtr0074214

NM_167491

2395

495

eas-RF

FBtr0308617

NM_001258777

4627

171

eas-RG

FBtr0308618

NM_001258778

2442

150

eas-RH

FBtr0308619

NM_176741

2401

495

eas-RI

FBtr0343519

NM_167489

2331

495

eas-RJ

FBtr0343520

NM_001298390

4575

171

Additional Transcript Data and Comments

Reported size (kB)

7, 5, 2.4 (northern blot)

(Pavlidis et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

eas-PA

FBpp0073991

56.8

495

5.02

NP_523364

AAF48574

eas-PB

FBpp0073990

56.8

495

5.02

NP_727942

AAN09387

eas-PC

FBpp0073993

56.8

495

5.02

NP_727943

AAF48575

eas-PF

FBpp0300841

19.2

171

8.93

FBpp0300842

17.2

150

9.97

NP_001245707

AFH07420

eas-PH

FBpp0300843

56.8

495

5.02

NP_788914

AAO41661

eas-PI

FBpp0310123

56.8

495

5.02

NP_727941

AAN09386

eas-PJ

FBpp0310124

19.2

171

8.93

X2JFQ7

NP_001285319

AHN59789

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

495 aa isoforms: eas-PA, eas-PB, eas-PC, eas-PH, eas-PI

171 aa isoforms: eas-PF, eas-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

517, 495 (aa)

(Pavlidis et al., 1994)

Comments

Activity assays show that eas protein is specific for ethanolamine and does not use choline as a substrate.

(Pavlidis et al., 1994)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Protein kinase-like domain superfamily

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eas using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

eas_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.92

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eas in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eas

View exonic expression by tissue for Dmel\eas

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32585/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000536

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000536

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000536

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0000536

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 27 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eas

Transgenic constructs containing regulatory region of eas

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(1)4b18

(Pascual et al., 2005, Pascual and Preat, 2001, Boquet et al., 2000, Rutherford, 1995)

Dp(1;4)81j6e

(Pavlidis et al., 1994)

Gene not disrupted in

Dp(1;4)exd^81h24b

(Rutherford, 1995, Pavlidis et al., 1994)

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eas^GD10683

viable, with Scer\GAL4^Mef2.PR

eas^GD10683

viable, with Scer\GAL4^pnr-MD237

eas^GD10683

viable, with Scer\GAL4^tin.CΔ4

eas^GD10683

Sterility

Allele

fertile

eas^EY01463

Other Phenotypes

Allele

abnormal behavior

abnormal behavior | recessive

abnormal flight

eas¹

abnormal memory

eas^unspecified

abnormal mitotic cell cycle

abnormal neuroanatomy

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.PFa

eas^KK102681

abnormal neurophysiology

abnormal neurophysiology | adult stage

abnormal size | adult stage, with Scer\GAL4^GMR.PFa

eas^KK102681

abnormal stress response

eas²

bang sensitive

bang sensitive | adult stage | heat sensitive

eas²

bang sensitive | recessive

paralytic

paralytic | chemical conditional

eas¹

paralytic | drug conditional

eas¹

wild-type

eas^hs.PP

Phenotype manifest in

Allele

adult heart

eas²

adult heart (with eas²)

eas^KG01772

adult heart (with eas^KG01772)

eas²

adult heart ventral longitudinal muscle cell

eas²

adult mushroom body alpha'-lobe

adult mushroom body alpha-lobe

adult mushroom body alpha-lobe (with Df(1)4b18)

eas^alaP

adult mushroom body beta'-lobe

adult mushroom body beta-lobe

adult mushroom body beta-lobe (with Df(1)4b18)

eas^alaP

axon | adult stage, with Scer\GAL4^GMR.PFa

eas^KK102681

calyx of adult mushroom body

eye photoreceptor cell | adult stage, with Scer\GAL4^GMR.PFa

eas^KK102681

female germline stem cell, with Scer\GAL4^Lsp2.3.1

mushroom body & neuron | somatic clone

eas^alaE13

optic cartridge | adult stage, with Scer\GAL4^GMR.PFa

eas^KK102681

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

Hsap\ETNK1

14 of 14

Yes

Hsap\ETNK2

13 of 14

Yes

Hsap\CHKA

3 of 14

Hsap\CHKB

3 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Etnk1

14 of 14

Yes

Rnor\Etnk2

13 of 14

Yes

Rnor\Chka

3 of 14

Rnor\Chkb

3 of 14

Mus musculus (laboratory mouse) (4)

Mmus\Etnk1

14 of 14

Yes

Mmus\Etnk2

12 of 14

Yes

Mmus\Chka

3 of 14

Mmus\Chkb

3 of 14

Xenopus tropicalis (Western clawed frog) (4)

Xtro\etnk1

5 of 13

Yes

Xtro\chka

2 of 13

Xtro\chkb

2 of 13

Xtro\ppfia1

1 of 13

Danio rerio (Zebrafish) (5)

Drer\etnk1

14 of 14

Yes

Drer\etnk2

13 of 14

Yes

Drer\chka

3 of 14

Drer\chkb

3 of 14

Drer\zgc:113516

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\ckc-1

13 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP000010

12 of 12

Yes

Agam\AgaP_AGAP007957

2 of 12

Arabidopsis thaliana (thale-cress) (4)

Atha\emb1187

12 of 13

Yes

Atha\AT1G74320

6 of 13

Atha\AT4G09760

6 of 13

Atha\CK1

6 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\CKI1

6 of 13

Yes

Scer\EKI1

6 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC13G7.12c

5 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\thiK

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:eas. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG2201

6 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 6 )

Allele

Disease

Evidence

References

eas¹

model of epilepsy

CEA

(Howlett and Tanouye, 2013, Whelan et al., 2010)

eas²

model of epilepsy

CEA

(Byers et al., 2021, Jeong et al., 2021, Jacobs and Sehgal, 2020, Cho et al., 2018, Saras and Tanouye, 2016, Kroll et al., 2015, Kroll and Tanouye, 2013, Hekmat-Scafe et al., 2010, Song et al., 2007)

model of heart conduction disease

CEA

(Lim et al., 2011)

CEA with eas^KG01772

(Lim et al., 2011)

eas^KG01772

model of heart conduction disease

CEA with eas²

(Lim et al., 2011)

eas^alaE13

model of epilepsy

CEA

(Moore and Taylor, 2023)

eas^unspecified

model of epilepsy

CEA

(Tan et al., 2004)

eas^2F

model of epilepsy

CEA

(Mituzaite et al., 2021)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

eas²

model of epilepsy

is ameliorated by shi^{ts1.IVS.p10.20xUAS}

(Kroll et al., 2015)

is ameliorated by shi¹

(Kroll et al., 2015)

is ameliorated by kcc^UAS.cHb

(Hekmat-Scafe et al., 2010)

is ameliorated by Top1¹¹²

(Song et al., 2007)

is ameliorated by Top1^JS

(Song et al., 2007)

is ameliorated by para^bss1

(Kroll and Tanouye, 2013)

is ameliorated by Mmp1^f1.UAS

(Cho et al., 2018)

is exacerbated by CG8839^KO

(Jacobs and Sehgal, 2020)

is ameliorated by wtrw^GD2850

(Jacobs and Sehgal, 2020)

is ameliorated by wtrw^KK103625

(Jacobs and Sehgal, 2020)

is ameliorated by wtrw^ex

(Jacobs and Sehgal, 2020)

is exacerbated by SLO2^CRISPR

(Byers et al., 2021)

is exacerbated by SLO2^DN.UAS

(Byers et al., 2021)

is ameliorated by Adcy1^rut-1

(Saras and Tanouye, 2016)

model of heart conduction disease

is ameliorated by Acc^B131

(Lim et al., 2011)

is ameliorated by FASN1^KK107330

(Lim et al., 2011)

is ameliorated by SREBP¹⁸⁹

(Lim et al., 2011)

is ameliorated by SREBP⁵²

(Lim et al., 2011)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ETNK1; ethanolamine kinase 1

14 of 14

609858

ETNK2; ethanolamine kinase 2

13 of 14

ETHANOLAMINE KINASE 2; ETNK2

CHKA; choline kinase alpha

3 of 14

118491

NEURODEVELOPMENTAL DISORDER WITH MICROCEPHALY, MOVEMENT ABNORMALITIES, AND SEIZURES; NEDMIMS

CHKB; choline kinase beta

3 of 14

612395

MUSCULAR DYSTROPHY, CONGENITAL, MEGACONIAL TYPE; MDCMC

megaconial type congenital muscular dystrophy

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lim et al., 2011)

suppressible

(Lim et al., 2011)

suppressible

(Saras and Tanouye, 2016)

eas

suppressible

EP2589

(Hekmat-Scafe et al., 2005)

eas

suppressible

esg

(Hekmat-Scafe et al., 2005)

suppressible

(Lim et al., 2011)

suppressible

(Muthukumar et al., 2014)

eas

suppressible

Itgbn

(Hekmat-Scafe et al., 2005)

eas

suppressible

kcc

(Hekmat-Scafe et al., 2010)

eas

suppressible

Mdr65

(Howlett and Tanouye, 2013)

eas

suppressible

mei-P26

(Glasscock et al., 2005)

eas

suppressible

mle|para

(Song and Tanouye, 2007)

eas

suppressible

pAbp

(Hekmat-Scafe et al., 2005)

eas

suppressible

para

(Song and Tanouye, 2007)

eas

suppressible

shakB

(Kuebler et al., 2001)

eas

suppressible

shi

(Kroll et al., 2015)

eas

enhanceable

SLO2

(Byers et al., 2021)

eas

suppressible

sna

(Hekmat-Scafe et al., 2005)

suppressible

(Lim et al., 2011)

suppressible

(Glasscock et al., 2005)

eas

suppressible

su(eas)13

(Glasscock et al., 2005)

eas

suppressible

tau

(Holth et al., 2013)

eas

suppressible

Top1

(Song et al., 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

kcc

enhanceable

eas

(Hekmat-Scafe et al., 2006)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58926

DroID - A comprehensive database of gene and protein interactions.

FBgn0000536

MIST (genetic) - An integrated Molecular Interaction Database

32585

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Synthesis of PE

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synthesis of PE

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-53

Cytogenetic map

14B7-14B7

Sequence location

X:16,278,328..16,285,618 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14B7-14B7

Limits computationally determined from genome sequence between P{EP}kat80^EP620&P{EP}caz^EP1564 and P{EP}Cyp1^EP1073&P{EP}Dsp1^EP355

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

14A-14B

(determined by in situ hybridisation)

(Boquet et al., 2000)

Experimentally Determined Recombination Data

Location

1-53

(FlyBase, 2016)

1-54.3

(Comeron et al., 2012)

1-53.5

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

9463

w¹¹¹⁸ eas^alaE13

10135

w¹¹¹⁸ P{EP}eas^EP770

9462

w¹¹¹⁸ P{GawB}eas^alaP

14246

y¹ P{SUPor-P}eas^KG01772

80159

w¹¹¹⁸; P{UAS-eas.P}3/TM3, Sb¹

43621

y¹ w^* Mi{MIC}eas^MI07243

Kyoto

125112

w¹¹¹⁸ P{RS5}eas^5-HA-1158

113240

w^* P{GawB}eas^NP3359 / FM7c

114111

w^* P{GawB}eas^NP7161 / FM7c

104604

y^* w^* P{GawB}eas^NP4102 / FM7c

VDRC

v103784

P{KK102681}VIE-260B

v34286

w¹¹¹⁸; P{GD10683}v34286

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (92)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH49854

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000536

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000536

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Mutants lack either alpha lobes or beta lobes of the mushroom body. Gamma lobes are normal. Learning and memory are normal.

(Pascual and Preat, 2001)

At temperatures ranging from 20^oC to 37.5^oC temperature sensitive mutants exhibit no effect on the heart rate of larvae.

(Dowse et al., 1995)

The eas bang sensitivity is due to an excitability defect caused by altered membrane lipid composition.

(Pavlidis et al., 1994)

Relationship to Other Genes

Source for database merge of

Source for merge of: eas ala

(Pascual et al., 2005)

Additional comments

Separable by recombination from bss (which causes similar phenotype), to which eas is closely linked.

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG3525

(Wang et al., 2019, Fukuoh et al., 2014, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Mohammad et al., 2009, Quinones-Coello, 2007, Bourbon et al., 2002, Dobrosotskaya et al., 2002, Morrison et al., 2000)

EP770

EtnK

(Malewicz et al., 1998)

PC80

RE10142

(Riedl et al., 2005)

RH11

ala

(Kristaponyte et al., 2012, Zars, 2010, Lu et al., 2009, van Swinderen, 2007, Margulies et al., 2005, Isabel et al., 2004, Heisenberg, 2003, Burrell and Sahley, 2001, Pascual and Preat, 2001, Pascual and Preat, 2001, Boquet et al., 2000)

eas

(Cuddapah et al., 2025, Karge et al., 2025, Fischer et al., 2024, Kasuya et al., 2023, Silva-Cardoso and N'Gouemo, 2023, Kelly et al., 2022, Majane et al., 2022, Saha et al., 2022, Banerjee et al., 2021, Byers et al., 2021, Lasko and Lüthy, 2021, Mituzaite et al., 2021, Ostalé et al., 2021, Dare et al., 2020, Hope et al., 2020, Jacobs and Sehgal, 2020, Martínez et al., 2020, Takai et al., 2020, Fogle et al., 2019, Kasuya et al., 2019, Radlicz et al., 2019, Tsai et al., 2019, Cho et al., 2018, Johan Arief et al., 2018, Reynolds, 2018, Sundararajan and Miller, 2018, Zhang et al., 2018, Li et al., 2017, Meltzer et al., 2017, Saras et al., 2017, Saras and Tanouye, 2016, Saras and Tanouye, 2016, Woinski and Reynolds, 2016, Di Cara et al., 2015, Kroll et al., 2015, Kroll et al., 2015, Ashwal-Fluss et al., 2014, Iyengar and Wu, 2014, Muthukumar et al., 2014, Toshima et al., 2014, Holth et al., 2013, Howlett and Tanouye, 2013, Howlett et al., 2013, Stone et al., 2013, Yin et al., 2013, Burg and Wu, 2012, Nesic et al., 2012, Winbush et al., 2012, Lim et al., 2011, Parker et al., 2011, Hekmat-Scafe et al., 2010, Kliman et al., 2010, Pauli et al., 2010, Whelan et al., 2010, Fergestad et al., 2008, Lim et al., 2008, Lindsay et al., 2008, Mensch et al., 2008, Song et al., 2008, Buszczak et al., 2007, Quinones-Coello, 2007, Reynolds et al., 2007, Song and Tanouye, 2007, Song et al., 2007, Fergestad et al., 2006, Hekmat-Scafe et al., 2006, Lee and Wu, 2006, Legube et al., 2006, Song and Tanouye, 2006, Reynolds et al., 2004, Tan et al., 2004, Kuebler et al., 2001, Nyako et al., 2001, Kuebler and Tanouye, 2000)

Name Synonyms

Easily shocked

(Johan Arief et al., 2018)

Ethanolamine kinase

(Dobrosotskaya et al., 2002, Dobrosotskaya et al., 2002, Malewicz et al., 1998)

alpha lobes absent

(Pascual and Preat, 2001)

alpha-lobe absent

(Zars, 2010)

easily-shocked

(Lim et al., 2008)

ethanolamine kinase

(Howlett et al., 2013)

α-lobe absent

(Heisenberg, 2003)

Secondary FlyBase IDs

FBgn0043963
FBgn0044449

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 72 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32585

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P54352

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P54352

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JFQ7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P54352

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32585/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000536

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000536

FlyMine - An integrated database for Drosophila genomics

FBgn0000536

InterPro - A database of protein families, domains and functional sites

Protein kinase-like domain superfamily

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3525

MARRVEL_MODEL - MARRVEL (model organism gene)

32585

Linkouts

BioGRID - A database of protein and genetic interactions.

58926

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000536

DroID - A comprehensive database of gene and protein interactions.

FBgn0000536

DRSC - Results frm RNAi screens

FBgn0000536

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

eas_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000536

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000536

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0000536

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0000536

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004370

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

32585

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synthesis of PE

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Synthesis of PE

References (184)

The following external sites may use different assemblies or annotations than FlyBase.

-0.92

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