FlyBase Allele Report: Dmel\kcc[UAS.cHb]

General Information

Symbol

Dmel\kcc^UAS.cHb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct b of Hekmat-Scafe

FlyBase ID

FBal0246596

Feature type

allele

Associated gene

Dmel\kcc

Associated Insertion(s)

Carried in Construct

P{UAS-kcc.B}

Also Known As

UAS-kcc

Key Links

Transgenic product class

cDNA

(Hekmat-Scafe et al., 2010)

wild_type

(Hekmat-Scafe et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hekmat-Scafe et al., 2010)

wild_type

(Hekmat-Scafe et al., 2010)

Mutagen

in vitro construct

(Hekmat-Scafe et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-kcc.B}

Cytology

Description

UASt regulatory sequences drive expression of the B isoform of kcc cDNA fragment from GH09271.

(Hekmat-Scafe et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kcc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by eas²

(Hekmat-Scafe et al., 2010)

modeled by para^bss1

(Hekmat-Scafe et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | first instar larval stage, with Scer\GAL4^naz.PS

(Parinejad et al., 2016)

abnormal neuroanatomy | first instar larval stage, with Scer\GAL4^naz.PS

(Parinejad et al., 2016)

Phenotype Manifest In

astrocyte-like glial cell | first instar larval stage, with Scer\GAL4^naz.PS

(Parinejad et al., 2016)

neuropil | first instar larval stage, with Scer\GAL4^naz.PS

(Parinejad et al., 2016)

Detailed Description

Statement

Reference

Expression of kcc^Scer\UAS.cHb under the control of Scer\GAL4^CG31235.PS leads to a reduction in astrocyte infiltration of the neuropil in L1 larvae, and reduced locomotor performance as compared to controls, with some larvae exhibiting paralysis and non-responsiveness to mechanical stimuli.

(Parinejad et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

kcc^UAS.cHb/Scer\GAL4^c739 is a suppressor of paralytic phenotype of para^bss1

(Hekmat-Scafe et al., 2010)

kcc^UAS.cHb/Scer\GAL4^c739 is a suppressor of paralytic phenotype of eas²

(Hekmat-Scafe et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^c739 significantly suppresses the bang-sensitive paralytic phenotype of eas² mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^c739 significantly suppresses the bang-sensitive paralytic phenotype of bss¹ mutants.

(Hekmat-Scafe et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

kcc^UAS.cHb/Scer\GAL4^elav.PLu partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^insc-Mz1407/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^ey-OK107/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^Alp4-c232/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^104y/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

kcc^UAS.cHb/Scer\GAL4^GH146 partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL80^Mef2.mb247, kcc^UAS.cHb, Scer\GAL4^elav.PLu partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^Mef2.247/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^c747/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

kcc^UAS.cHb/Scer\GAL4^c739 partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^c305a/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^Tab2-201Y/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^Rdl.PS/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Scer\GAL4^Gad1.3.098/kcc^UAS.cHb partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

kcc^UAS.cHb/Scer\GAL4^ChAT.7.4 partially rescues kcc^DHS1

(Hekmat-Scafe et al., 2010)

Fails to rescue

Scer\GAL4^repo.PU/kcc^UAS.cHb fails to rescue kcc^DHS1

(Hekmat-Scafe et al., 2010)

Comments

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^repo.PU produces no apparent change in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^elav.PLu results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4¹⁴⁰⁷ results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^ey-OK107 results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^Aph-4-c232 results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^104Y results only in a modest reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^GH146 results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Co-expression of Scer\GAL80^Mef2.PT with Scer\GAL4^elav.PLu-driven kcc^Scer\UAS.cHb results in a significantly compromise in the rescuing ability of kcc^Scer\UAS.cHb. Scer\GAL80^Mef2.PT co-expression significantly compromises the ability of Scer\GAL4^elav.PLu-driven kcc^Scer\UAS.cHb to rescue the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^Mef2.247 results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^c747 results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants. Electrophysiology shows that Scer\GAL4^c747-driven kcc^Scer\UAS.cHb expression raises the seizure threshold of kcc^DHS1 mutant flies.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^c739 results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^c305a results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^Tab2-201Y results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^Rdl.PS results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^Gad1.3.098 results in a significant reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

Expression of kcc^Scer\UAS.cHb driven by Scer\GAL4^Cha.7.4 results only in a modest reduction in the bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants.

(Hekmat-Scafe et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kcc^Scer\UAS.cHb

kcc^UAS.cHb

Name Synonyms

Saccharomyces cerevisiae UAS construct b of Hekmat-Scafe

Secondary FlyBase IDs

References (4)

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