FlyBase Allele Report: Dmel\Top1[JS]

General Information

Symbol

Dmel\Top1^JS

Species

D. melanogaster

Name

FlyBase ID

FBal0240903

Feature type

allele

Associated gene

Dmel\Top1

Associated Insertion(s)

P{lacW}Top1^JS

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Song et al., 2007)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{lacW}Top1^JS

Cytology

Description

P{lacW} is inserted in the 5' UTR of the Top1 gene, 257bp upstream of the translational start site of isoforms A and C (256075/256076 of AE003498).

(Song et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by eas²

(Song et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | recessive

(Song et al., 2007)

viable

(Song et al., 2007)

viable (with Top1⁷⁷)

(Song et al., 2007)

viable (with Top1¹¹²)

(Song et al., 2007)

viable (with Top1^G0134)

(Song et al., 2007)

viable (with Top1^G0201)

(Song et al., 2007)

viable (with Top1^G0229)

(Song et al., 2007)

Phenotype Manifest In

adult brain

(Song et al., 2007)

Detailed Description

Statement

Reference

Top1^JS is hemizygous and homozygous viable. Top1^JS hemizygous males are not bang sensitive and have a wild-type seizure threshold*. However, these flies have increased levels of apoptosis in the adult brain. (*seizure threshold = minimum voltage of high frequency stimulation required to induce seizures).

(Song et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Top1^G0229/Top1^JS is a suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Top1¹¹²/Top1^JS is a suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Df(1)ED7294/Top1^JS is a suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Top1^JS is a suppressor of bang sensitive | recessive phenotype of jus^iso7.8

(Song et al., 2007)

Top1¹¹²/Top1^JS is a suppressor of bang sensitive | recessive phenotype of jus^iso7.8

(Song et al., 2007)

Top1^JS is a suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Top1^JS/Top1^JS is a suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

NOT Suppressor of

Statement

Reference

Top1^+t10/Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Scer\GAL4^elav-C155, Top1^JS, Diap1^UAS.cHa is a non-suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Top1^G0134/Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Top1^G0201/Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of eas²

(Song et al., 2007)

Top1^G0229/Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of jus^iso7.8

(Song et al., 2007)

Top1^G0201/Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of jus^iso7.8

(Song et al., 2007)

Top1^G0134/Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of jus^iso7.8

(Song et al., 2007)

Top1^JS is a non-suppressor of bang sensitive | recessive phenotype of para^unspecified

(Song et al., 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The bang sensitive phenotype and decreased seizure threshold* of eas² homozygotes are partially suppressed by Top1^JS/Y, Top1^JS/Top1^G0229, Top1^JS/Top1¹¹² or Top1^JS/Df(1)ED7294 but not by Top1^JS/Top1^G0201, Top1^JS/Top1^G0134 or Top1^JS/Y; Top1^+t10. Top1^JS/Y also reduces the time required to recover after seizure in eas² homozygotes. The partial suppression of the bang sensitive phenotype and decreased seizure threshold* of eas² homozygotes is itself suppressed by th^Scer\UAS.cHa, Scer\GAL4^elav-C155. (*seizure threshold = minimum voltage of high frequency stimulation required to induce seizures).

The bang sensitive phenotype and decreased seizure threshold of sda^iso7.8 homozygotes is partially suppressed by Top1^JS/Y or Top1^JS/Top1¹¹², but not by Top1^JS/Top1^G0229, Top1^JS/Top1^G0201 or Top1^JS/Top1^G0134.

The bang sensitive phenotype and decreased seizure threshold of bss^unspecified is not suppressed by Top1^JS/Y, however, the increased recovery time after seizure seen in these flies is partially suppressed by Top1^JS/Y.

(Song et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Top1^G0134

(Song et al., 2007)

Top1^G0201

(Song et al., 2007)

Top1^G0229

(Song et al., 2007)