Imprecise excision of the progenitor insertion has resulted in a deletion which affects both Gyc76C and HLH106 (neighbouring genes). The deletion extends 697bp into the HLH106 open reading frame, up to amino acid residue 233 in exon 3 (the next in-frame start codon is at amino acid residue 265). The deletion also removes the predicted first exon of Gyc76C.
On unsupplemented medium, S2PKG08356/S2P1 mutants in the presence of two copies of HLH106D386A and carrying HLH106189/+, survive at less than 25% of the expected rate. When these animals are homozygous for HLH106189, survival at only 10% of the expected rate is observed. Survival is restored by supplementing the culture medium with free fatty acids.