FlyBase Allele Report: Dmel\S2P[KG08356]

General Information

Symbol

Dmel\S2P^KG08356

Species

D. melanogaster

Name

FlyBase ID

FBal0147528

Feature type

allele

Associated gene

Dmel\S2P

Associated Insertion(s)

P{SUPor-P}S2P^KG08356

Carried in Construct

Also Known As

dS2P²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}S2P^KG08356

Cytology

Description

P{SUPor-P}S2P^KG08356 insertion disrupts the open reading frame of the S2P transcript at codon 261. An in-frame stop codon arises from P-element sequences four codons after the insertion site.

(Matthews et al., 2009)

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:11,843,061..11,843,061 [-]

(Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size (with S2P¹)

(Matthews et al., 2009)

lethal - all die before end of larval stage (with S2P¹)

(Amarneh et al., 2009)

some die during larval stage (with S2P¹)

(Amarneh et al., 2009)

viable | conditional

(Matthews et al., 2009)

viable | conditional (with S2P¹)

(Amarneh et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Viability is reduced in S2P^KG08356/S2P¹ mutant flies from S2P^KG08356/S2P^KG08356 homozygous mothers mated to S2P¹ males. Viability is restored by feeding the flies medium enriched with fatty acids.

(Amarneh et al., 2009)

S2P^KG08356/S2P¹ larvae are comparable in size to control larvae 48 hours after egg laying. However, from this age mutant larvae are distinctly smaller than control larvae, and emerge on average 2 days late (or even later if animals are raised in conditions of crowding). By adulthood, mutants display a greater variability in body size compared to controls. Mutants show normal morphology.

Viability is reduced in S2P^KG08356/S2P¹ mutant flies from S2P^KG08356/S2P^KG08356 homozygous mothers mated to S2P¹ males, compared to progeny from heterozygous mutant parents. This reduced viability is restored by feeding the flies on a fatty-acid enriched culture medium.

(Matthews et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

S2P^KG08356/S2P¹ has lethal | larval stage phenotype, enhanceable by HLH106[+]/SREBP¹⁸⁹, SREBP^D386A/SREBP^D386A

(Amarneh et al., 2009)

S2P^KG08356/S2P¹ has lethal | larval stage phenotype, enhanceable by SREBP^D386A/SREBP^D386A/SREBP¹⁸⁹/SREBP¹⁸⁹

(Amarneh et al., 2009)

Suppressed by

Statement

Reference

S2P^KG08356/S2P¹ has viable | conditional phenotype, suppressible by Drice^Δ1/Ice[+]

(Amarneh et al., 2009)

S2P^KG08356/S2P¹ has viable | conditional phenotype, suppressible by Drice^Δ1/Drice^Δ1

(Amarneh et al., 2009)

S2P^KG08356/S2P¹ has viable | conditional phenotype, suppressible by Dronc⁵¹/Nc[+]

(Amarneh et al., 2009)

S2P^KG08356/S2P¹ has viable | conditional phenotype, suppressible by Dronc⁵¹/Dronc⁵¹

(Amarneh et al., 2009)

NOT suppressed by

Statement

Reference

S2P^KG08356/S2P¹ has viable | conditional phenotype, non-suppressible by HLH106[+]/SREBP¹⁸⁹, SREBP^D386A/SREBP^D386A

(Amarneh et al., 2009)

S2P^KG08356/S2P¹ has viable | conditional phenotype, non-suppressible by SREBP^D386A/SREBP^D386A/SREBP¹⁸⁹/SREBP¹⁸⁹

(Amarneh et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

S2P^KG08356/S2P¹, Ice^Δ1/+ and S2P^KG08356/S2P¹, Ice^Δ1/Ice^Δ1 mutant larvae exhibit lethality between second and third instar stages. Lethality is rescued partially by supplementing fly food with fatty acids, but viability is still reduced compared to S2P^KG08356/S2P¹ mutants.

S2P^KG08356/S2P¹, Nc⁵¹/+ and S2P^KG08356/S2P¹, Nc⁵¹/Nc⁵¹ mutant larvae exhibit lethality between second and third instar stages. Lethality is rescued partially by supplementing fly food with fatty acids, but viability is still reduced compared to S2P^KG08356/S2P¹ mutants.

On unsupplemented medium, S2P^KG08356/S2P¹ mutants in the presence of two copies of HLH106^D386A and carrying HLH106¹⁸⁹/+, survive at less than 25% of the expected rate. When these animals are homozygous for HLH106¹⁸⁹, survival at only 10% of the expected rate is observed. Survival is restored by supplementing the culture medium with free fatty acids.

(Amarneh et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

14717

y¹ w^67c23; P{SUPor-P}S2P^KG08356

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

KG08356

(Matthews et al., 2009)

S2P^KG08356

dS2P²

(Amarneh et al., 2009, Matthews et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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