A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Df(3L)BSC845 fails to complement Df(3L)BSC442 and Df(3L)BSC558.
Inferred to overlap with: Df(3L)BSC442.
Inferred to overlap with: Df(3L)BSC558.
The presence of P+PBac{XP5.RB3}BSC845 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in FBrf0174229.
The breakpoints of Df(3L)BSC845 predicted from the Release 5 genomic coordinates of the progenitor P{XP}d08056 and PBac{RB}Dcp2e00034 transposable element insertion sites are 3L:15504128 ;15819023 and the cytological breakpoints predicted from these coordinates are 71D3;72A1.