The imprecise excision of P{EPgy2}EY21985 results in a 924 bp deletion in the regulatory regions of Nph and Nlp that also extends into the coding region of Nlp.
Df(3R)Nlp-Nph homozygotes and Df(3R)Nlp-Nph/Df(3R)Exel6213 animals are viable and fertile.