Homozygotes survive at 18^oC.

Very weak suppressor of In(1)w^m4h variegation.

8.5% of homozygotes survive to adulthood at 18^oC, 6.8% survive to adulthood at 25^oC, and no homozygotes survive to adulthood at 29^oC.

Homozygous males raised at 18^oC appear to be sluggish in their movements and fail to copulate with wild-type females in a courtship assay. Homozygous female virgins raised at 18^oC fail to copulate with wild-type males in a courtship assay.

Homozygous adults which have been raised at 18^oC and then stored for 3-5 days at that temperature pass out irreversibly (and possibly die immediately) within 61 +/- 2 minutes when subjected to 33^oC.

Ace^j40/Ace^j19 or Ace^j40/Ace^j50 second and third instar larvae which have been raised at 18^oC are no more susceptible to heat-induced paralysis when exposed to 38^oC than control larvae, and are if anything, less adversely affected.

Ace^j40/Ace^j19 or Ace^j40/Ace^j50 adults which have been raised at 18^oC show a slight defects in phototactic behaviour when tested at 18^oC. The defects in phototaxis increase if the flies are subjected to 29^oC.

Ace^j40/Ace^j19 or Ace^j40/Ace^j50 adults which have been raised at 18^oC but subjected to a heat pulse during pupal development show severely defective phototaxis, while heat treatment during larval development results in only a slight decrement in phototaxis in the resulting adults.

Ace^j40/Ace^j50 adults which have been raised at 18^oC but subjected to a heat pulse during pupal development show a severe reduction in general locomotor activity compared to mutant adults of the same genotype that have been maintained at 18^oC throughout development.

Ace^j40/Ace^j50 adults which have been raised at 18^oC and then shifted to 29^oC for 11 days show defects in the neuropile of the brain; it appears compacted and disorganised compared to wild type.

2% survival in combination with Df(3R)l26d at 18^oC, none at 29^oC. Exposure of Ace^j40/Ace^j19 or Ace^j40/Ace^j50 flies to restrictive temperature during late embryonic-early larval stage is lethal; little effect on mid and late larval stages; pupal exposure causes defects in adult phototaxis and motor activity. Heat treatment of adults causes no decline in ACE activity but decrements in phototaxis (29^oC) and cessation of movement (31^oC) observed.