FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Act88F8
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General Information
Symbol
Dmel\Act88F8
Species
D. melanogaster
Name
FlyBase ID
FBal0000274
Feature type
allele
Associated gene
Dmel\Act88F
Associated Insertion(s)
Carried in Construct
Also Known As
rsd
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

Mutagen
spontaneous
Progenitor genotype
Cytology
Description

Alteration of normal sequence apparently outside the coding region; Sequence of coding region of DNA including 60-nucleotide intron reveals differences from that of Canton-S that account for five amino-acids substitution.

(Mahaffey et al., 1985)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Heterozygotes are flightless with a normal wing posture at 22oC, but are capable of hopping and flying short distances at 29oC. Homozygotes are flightless with an abnormal wing posture.

      (Homyk and Emerson, 1988)

      Electron microscropy of homozygotes reveals grossly abnormal indirect-flight-muscle structure; lack thin filaments and Z discs.

      (Deak et al., 1982)

      Wings of homozygotes held straight up, nearly meeting over thorax; heterozygotes have wings held normally, but are nearly flightless.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      Act88F8, up101 has visible | dominant | cold sensitive phenotype

      (Homyk and Emerson, 1988)

      Act88F8, rsd1, up101 has visible | dominant phenotype

      (Homyk and Emerson, 1988)

      Act88F8, Mhc6 has visible | dominant phenotype

      (Homyk and Emerson, 1988)

      Act88F8, up2 has visible | dominant phenotype

      (Homyk and Emerson, 1988)

      Act88F8, rsd1 has viable phenotype

      Act88F8, rsd1 has fertile phenotype

      Act88F8, rsd1 has flightless | dominant phenotype

      Phenotype Manifest In
      Other
      Statement
      Reference

      Act88F8, up101 has wing phenotype

      (Homyk and Emerson, 1988)

      Act88F8, Mhc6 has wing phenotype

      (Homyk and Emerson, 1988)

      Act88F8, up2 has wing phenotype

      (Homyk and Emerson, 1988)

      Act88F8, rsd1 has indirect flight muscle cell phenotype

      Act88F8, rsd1 has wing phenotype

      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Some double heterozygotes with Mhc6 or up2 have an abnormal wing posture. Double heterozygotes with up101 have an abnormal wing posture at 22oC.

      (Homyk and Emerson, 1988)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Studied only in combination with rsd; not examined in rsd+ background. Conceivable that Act88F8 is a wild-type isoallele with normal phenotype in the absence of rsd1.

      Abnormal protein accumulation observed in thoraces. Actin III and its ubiquitinated derivative, arthrin, absent in Act88F8 homozygotes; six other polypeptides, including an indirect-flight-muscle tropomyosin isoform and two indirect-flight-muscle tropomyosin-related isoforms, markedly reduced. Homozygotes transformed with Act88F+ show restoration to approximately normal levels of the six reduced polypeptides. Accumulation of actin III and arthrin still negative, however; the latter attributed to the failure of post-translational modification in the presence of homozygous rsd1.

      (Lang et al., 1981)

      Act88F mRNA reduced 10-15 fold; mRNA level and to some degree, the phenotype rescuable by germ-line transformation using Act88F normal genomic sequence.

      (Mahaffey et al., 1985)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Act88F8
      Act88Frsd
      rsd
      (Homyk and Emerson, 1988, Deak et al., 1982)
      Name Synonyms
      raised
      (Fyrberg et al., 1998)
      Secondary FlyBase IDs
        References (6)