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General Information
Symbol
Dmel\Adhn1
Species
D. melanogaster
Name
negative
FlyBase ID
FBal0000332
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G14616681A

Reported nucleotide change:

G1120A

Amino acid change:

G93E | Adh-PC; G93E | Adh-PE; G93E | Adh-PF; G93E | Adh-PH; G93E | Adh-PI

Reported amino acid change:

G92E

Comment:

Induced on an AdhS chromosome.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G93E. Nucleotide substitution: G1120A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies of the transheterozygous mutant genotypes Adhn1/Adhfn6 and Adhn1/Adhfn23 both show the same defective phenotypic response to ethanol. These flies experience a hyperactive phase that is of a much lower magnitude than in wild type. The proportion of akinetic flies is substantially higher amongst the Adh mutants than in wild-type flies and the mutants accumulate higher amounts of ethanol. However, the mutants have a normal startle response to ethanol.

Sensitive to ethanol.

Adhn1 larvae have the same resistance to anoxia as wild-type larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (6)
Notes on Origin
Discoverer

E.H. Grell.

Comments
Comments

Enzyme activity: 20% of wild type progenitor. CRM positive. Forms active hybrid enzyme. Polypeptide slightly larger than wild type on SDS gels and electrophoretic mobility altered on non-denaturing gels.

Ethanol concentration of 3.5% and lower does not cause any additional mortality of Adh- eggs compared to wild type. If 2 hour eggs are subjected to 3.5% ethanol their mortality is higher than for 9 and 18 hour eggs. It is postulated that the membrane is not fully developed at this stage so the eggs are more vulnerable. Survival is less than 5% on ethanol concentrations of 4.3% and 4.5%. Eggs brought into contact with this concentration of ethanol show a higher viability than eggs at a later stage due to a phenotypic increase in ethanol tolerance.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
negative
Secondary FlyBase IDs
    References (15)