hypomorphic allele - genetic evidence
amorphic allele - genetic evidence
Product forms active hybrid enzyme.
Purified enzyme thermolabile; Adhn5 flies grown at 18oC show loss of both ADH activity and CRM following a shift to 30oC (Tsubota, quoted in FBrf0066905); recovery on return to 18oC takes several days.
Gel assays demonstrate that the Adhn5 protein has an acidic charge relative to the wild type Adh protein and cannot form aggregates.