Mad^k00237/Mad^apg larvae transferred to 29^oC at the equivalent of 48 hours, 72 hours, 84 hours or 96 hours after egg laying develop at the same rate as wild-type siblings.

Removal of Mad function before 72 hours after egg laying in Mad^apg/Mad^k00237 flies produces truncations of the proximal-distal axis in the legs, whereas removal of Mad function from 84 hours after egg laying until the end of development results in a normal proximal-distal organisation in the leg despite dorsal patterning defects.

Homozygotes when raised at 19^oC show occasional absence of one or both tarsal claws. Homozygotes are fertile at 19^oC but become sterile when shifted to 29^oC, laying fertile eggs which do not hatch, the unhatched embryos having a range of germ band abnormalities. Homozygous pupal lethal when raised at 28^oC, pharate adults show defective tarsal development of all six legs, having a condensed, poorly developed and curved metatarsus and tarsi, duplications in tibial and tarsal segments and lack tarsal claws. Temperature sensitive period is first instar to early pupa. Veins L4 and L5 are interrupted and abdominal segments are partially fused (these phenotypes are not temperature sensitive).