Oogenesis arrests at the germarial stage in S¹ aret^PD/Df(2L)esc-P2-0 females. The ovarioles fail to bud off into individual egg chambers

and instead, multiple undifferentiated germ cells appear in the severely

truncated ovarioles.

Oogenesis arrests at stage 6/7 in S¹ aret^PA/aret^PD females.

Oogenesis arrests at about stage 3 in S¹ aret^PD/aret^QB

females and no oocyte is specified.

The oogenesis defects of S¹ aret^PD/Df(2L)esc-P2-0 females

are partially suppressed by one copy of Df(3R)M-Kx1; egg chambers

bud off from the germarium, although they are abnormal - each egg chamber

has more than the normal number of 16 germ cells and no oocyte is specified.

Df(3R)Dl-BX12 dominantly alters the ovary phenotype of S¹ aret^PD/Df(2L)esc-P2-0

females; the germarium is greatly expanded to produce a large volume

of germ cells surrounded by a layer of follicle cells.

The oogenesis arrest phenotype of S¹ aret^PD/Df(2L)esc-P2-0

females is suppressed by Df(3R)mbc-R1/+.

Df(3R)M-Kx1 dominantly suppresses the ovary phenotype of aret^PD/aret^QB

females; the arrest in oogenesis occurs later in the double mutants

than in the single mutant females.

Expression of Lk6^EP886 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}

enhances the ovary phenotype of aret^PD/aret^QB females (oogenesis

arrests at 3), while in the absence of an Scer\GAL4 driver, Lk6^EP886

suppresses the ovary phenotype of aret^PD/aret^QB females (oogenesis

arrests at stage 9).

The hatch rate of embryos derived from aret^PA/aret^PD females

is increased from 3% to 14% in the presence of Lk6^EP886/+.

aret^PD/aret^QB eIF-4E^S251D ; Lk6^EP886/+ females arrest

oogenesis at the same stage (stage 9) as aret^PD/aret^QB ; Lk6^EP886/+

females.

eIF-4E^S251D suppresses the aret^PD/aret^QB ovary phenotype,

delaying arrest of oogenesis until stage 9.

Most egg chambers have more than 16 germline cells in aret^QB/aret^PD

; Dl^9P/+ ovaries. Three classes of abnormal egg chambers are seen,

which are present in roughly equal numbers. In the first class, a

large number of germline cells of roughly equal size are enveloped

by a single epithelium of follicle cells. The remaining two classes

arise from partial fusion of separate cysts; no stalk cells can be

detected and the follicle cell layers of different egg chambers remain

in contact with each other. In the "anterior/posterior fusion" class,

a well-defined linear organisation within individual ovarioles is maintained,

and adjacent egg chambers are fused with each other at their anterior

and posterior boundaries. In the "random fusion" class, egg chambers

are positioned irregularly and can be closely apposed to multiple different

egg chambers on lateral as well as on anterior and posterior surfaces.

In both the partial fusion classes, each egg chamber contains an oocyte.

In the anterior/posterior fusion class the oocyte is present at the

posterior of the cyst and in the random fusion class the oocyte is

either lateral or posterior relative to the overall polarity of the

ovariole.