FlyBase Allele Report: Dmel\bru1[PA]

General Information

Symbol

Dmel\bru1^PA

Species

D. melanogaster

Name

FlyBase ID

FBal0000703

Feature type

allele

Associated gene

Dmel\bru1

Associated Insertion(s)

Carried in Construct

Also Known As

aret^PA, aret^PA62

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Sugimura and Lilly, 2006)

Mutagen

ethyl methanesulfonate

(Webster et al., 1997, Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement: H213P. H213P falls in the first RNA-binding domain.

(Webster et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:12,278,897..12,278,897 [+]

Nucleotide change:

A12278897C

Amino acid change:

Reported amino acid change:

H213P

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Webster et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with bru1^QB)

(Sugimura and Lilly, 2006)

abnormal mitotic cell cycle (with bru1^WH)

(Sugimura and Lilly, 2006)

abnormal mitotic cell cycle (with bru1^Z2286)

(Sugimura and Lilly, 2006)

female sterile

(Schupbach and Wieschaus, 1991)

female sterile (with bru1^PD)

(Spletter et al., 2015)

female sterile (with bru1^QB)

(Spletter et al., 2015)

female sterile | recessive

flightless (with bru1^PD)

(Spletter et al., 2015)

flightless (with bru1^QB)

(Spletter et al., 2015)

viable (with bru1^PD)

(Spletter et al., 2015)

viable (with bru1^QB)

(Spletter et al., 2015)

Phenotype Manifest In

egg chamber

(Webster et al., 1997)

embryonic/first instar larval cuticle | maternal effect (with bru1^PD)

(Webster et al., 1997)

mitotic spindle | ectopic | oogenesis stage S4 (with bru1^QB)

(Sugimura and Lilly, 2006)

mitotic spindle | ectopic | oogenesis stage S4 (with bru1^WH)

(Sugimura and Lilly, 2006)

mitotic spindle | ectopic | oogenesis stage S4 (with bru1^Z2286)

(Sugimura and Lilly, 2006)

nurse cell (with bru1^QB)

(Sugimura and Lilly, 2006)

nurse cell (with bru1^WH)

(Sugimura and Lilly, 2006)

nurse cell | oogenesis stage S4 (with bru1^QB)

(Sugimura and Lilly, 2006)

nurse cell | oogenesis stage S4 (with bru1^WH)

(Sugimura and Lilly, 2006)

nurse cell | oogenesis stage S4 (with bru1^Z2286)

(Sugimura and Lilly, 2006)

ovary

(Parisi et al., 2001)

ovary (with bru1^PD)

(Yan and Macdonald, 2004)

Detailed Description

Statement

Reference

In aret^PA/aret^WH, aret^PA/aret^QB, or aret^PA/aret^Z2286 adult females, oogenesis proceeds normally until stage 4 - meiosis and mitosis of germ cells occurs normally and nurse cells become enlarged an polyploid. But during stage 4, when mitosis of germ cells is over in wild-type, in around 20% of cysts the nurse cells condense their chromosomes and form large mitotic spindles.

(Sugimura and Lilly, 2006)

Nuage does not appear disrupted in aret^PA/aret^PD nurse cells.

(Snee and Macdonald, 2004)

aret^PA/aret^PD females have a weak ovarian phenotype; many egg chambers produce mature oocytes that can be fertilised and progress through much of embryonic development. Only 3% of embryos derived from these females hatch.

(Yan and Macdonald, 2004)

Mutants show a oogenic phenotype.

(Parisi et al., 2001)

Oogenesis proceeds normally until approximately stage 9, when the egg chambers deteriorate, in hemizygous females. No late stage eggs are formed or laid. aret^PA/aret^PD females complete oogenesis and lay eggs, some of which hatch into viable larvae. However, the majority of embryos derived from these females have complex cuticle defects involving partial or complete fusion of adjacent segments.

(Webster et al., 1997)

Almost normal numbers of egg chambers that degenerate early.

(Schupbach and Wieschaus, 1991)

Homozygous females usually have almost normal numbers of early stages of egg chambers in their ovaries which degenerated before yolk uptake occurs.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

bru1^PA/bru1^Z2286, mr¹/mr² has abnormal mitotic cell cycle | oogenesis stage S2 phenotype

(Sugimura and Lilly, 2006)

bru1^PA/bru1^Z2286, mr¹/mr² has abnormal mitotic cell cycle | oogenesis stage S1 phenotype

(Sugimura and Lilly, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

bru1^PA/bru1^PD has ovary phenotype, enhanceable by S[+]/S¹

(Yan and Macdonald, 2004)

Suppressed by

Statement

Reference

bru1^QB/bru1^PA has phenotype, suppressible by Df(3R)M-Kx1

(Yan and Macdonald, 2004)

Enhancer of

Statement

Reference

bru1^PA is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

Other

Statement

Reference

bru1^PA/bru1^Z2286, mr¹/mr² has germline cell | female | oogenesis stage S1 phenotype

(Sugimura and Lilly, 2006)

bru1^PA/bru1^Z2286, mr¹/mr² has germline cell | female | oogenesis stage S2 phenotype

(Sugimura and Lilly, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Oogenesis arrests at stage 6/7 in S¹ aret^PA/aret^PD females. The hatch rate of embryos derived from aret^PA/aret^PD females is increased from 3% to 14% in the presence of Lk6^EP886/+.

(Yan and Macdonald, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

aret^P62A

(Schupbach and Wieschaus, 1991)

aret^PA62

(Moore et al., 2009, Vicente-Crespo et al., 2008, Parisi et al., 2001, Webster et al., 1997)

aret^PA

(Flora et al., 2018, Davidson et al., 2016, Kanke et al., 2015, Spletter et al., 2015, Tadros et al., 2007, Sugimura and Lilly, 2006, Snee and Macdonald, 2004)

aretPA62

(Bose et al., 2022)

arrest^PA62

(Jankovics et al., 2001)

bru1^PA

Name Synonyms

Secondary FlyBase IDs

References (15)

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