FlyBase Allele Report: Dmel\mr[1]

General Information

Symbol

Dmel\mr¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012432

Feature type

allele

Associated gene

Dmel\mr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W739R.

(Kashevsky et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,975,146..23,975,146 [+]

Nucleotide change:

T23975146C

Amino acid change:

W739R | mr-PA

Reported amino acid change:

W739R

Comment:

Same mutation as mr². Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kashevsky et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Reed and Orr-Weaver, 1997)

partially lethal (with mr³)

(Kashevsky et al., 2002)

sterile (with mr²)

(Kashevsky et al., 2002)

Phenotype Manifest In

abdominal tergite

(Reed and Orr-Weaver, 1997)

egg

(Reed and Orr-Weaver, 1997)

embryo

(Reed and Orr-Weaver, 1997)

eye

(Reed and Orr-Weaver, 1997)

mitotic cell cycle | embryonic stage

(Reed and Orr-Weaver, 1997)

nuclear chromosome | maternal effect (with mr²)

(Kashevsky et al., 2002)

nurse cell

(Kashevsky et al., 2002)

nurse cell (with mr²)

(Kashevsky et al., 2002)

spindle | embryonic stage

(Reed and Orr-Weaver, 1997)

spindle | maternal effect (with mr²)

(Kashevsky et al., 2002)

Detailed Description

Statement

Reference

mr¹/mr² females show no apparent defect in germline stem cell maintenance.

(Chen et al., 2009)

The nurse cells revert to mitosis at stage 5 in homozygous and mr¹/mr² egg chambers. Nurse cells in later mr¹/mr² egg chambers often become pycnotic. Embryos derived from mr¹/mr² egg chambers which complete oogenesis complete meiosis, however, the zygotic nuclei and frequently the polar bodies, are arrested on metaphase spindles that are anastral or have broad poles. The chromosomes are hypercondensed in these embryos.

(Kashevsky et al., 2002)

Semi-lethal when hemizygous, with most hemizygotes dying as pharate adults. Escapers have etched tergites and very rough eyes. mr¹/mr² females sometimes lay eggs (which do not hatch). Fertilised eggs show an early metaphase arrest phenotype, containing usually between 6 and 10 metaphase nuclei. The spindles are broader than wild-type both at the metaphase plate and at the spindle poles.

(Reed and Orr-Weaver, 1997)

Eyes rough. Bristles irregularly reduced in size and number. Abdominal sclerites often smaller. Developmental study by Lees and Waddington (1942) shows that effect on bristles results from general slowing of bristle growth. Female entirely sterile, has underdeveloped ovaries. At 19^oC, bristles nearly normal and eyes nearly wild type. RK2 at 25^oC and above.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mr¹/mr³ has partially lethal phenotype, enhanceable by CycB^+t10

(Kashevsky et al., 2002)

Suppressor of

Statement

Reference

mr[+]/mr¹ is a suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Other

Statement

Reference

bru1^PA/bru1^Z2286, mr¹/mr² has abnormal mitotic cell cycle | oogenesis stage S2 phenotype

(Sugimura and Lilly, 2006)

bru1^PA/bru1^Z2286, mr¹/mr² has abnormal mitotic cell cycle | oogenesis stage S1 phenotype

(Sugimura and Lilly, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

mr¹ has phenotype, enhanceable by e(mr)¹

(Reed, 1992)

NOT suppressed by

Statement

Reference

mr¹ has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

mr¹/mr² is an enhancer of female germline stem cell phenotype of eff^mer1/eff⁸

(Chen et al., 2009)

Suppressor of

Statement

Reference

mr[+]/mr¹ is a suppressor of plasma membrane | oogenesis phenotype of vih^ΔN-9

(Braun et al., 2021)

mr[+]/mr¹ is a suppressor of female germline cyst phenotype of vih^ΔN-9

(Braun et al., 2021)

mr[+]/mr¹ is a suppressor of centrosome | oogenesis phenotype of vih^ΔN-9

(Braun et al., 2021)

mr[+]/mr¹ is a suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Other

Statement

Reference

bru1^PA/bru1^Z2286, mr¹/mr² has germline cell | female | oogenesis stage S1 phenotype

(Sugimura and Lilly, 2006)

bru1^PA/bru1^Z2286, mr¹/mr² has germline cell | female | oogenesis stage S2 phenotype

(Sugimura and Lilly, 2006)

e(mr)¹, mr¹/mr² has antennal lobe phenotype

(Reed, 1992)

Additional Comments

Genetic Interactions

Statement

Reference

mr¹/mr² enhances the loss of germline stem cells that is seen in eff⁸/eff^mer1 females, such that the rate of germline stem cell loss is more rapid in the double mutant females.

(Chen et al., 2009)

In aret^PA/aret^Z2286 ; mr¹/mr² double mutant adult female, germ cells show a high frequency of metaphase arrest beginning during stage 1-2 of oogenesis.

(Sugimura and Lilly, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mr¹/mr² is rescued by Scer\GAL4^Act5C.PI/mr^UASp.cKa

(Kashevsky et al., 2002)

mr¹/mr² is rescued by Scer\GAL4^{VP16.nanos.UTR}/mr^UASp.cKa

(Kashevsky et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

817

In(2L)NS, In(2R)NS, Adc^b-1 l(2)35De¹ mr¹/In(2L)Cy, In(2R)Cy, Duox^Cy

380

px¹ bw¹ mr¹ speck¹/In(2LR)bw^V1, ds^33k bw^V1

Kyoto

101509

In(2L)Cy In(2R)Cy, Duox^Cy cn² / In(2L)NS In(2R)NS, Adc^b-1 mr¹

Notes on Origin

Discoverer

Comments

Germ-line clonal analysis indicates that the mr¹ nurse cell phenotype is likely to be germ-line-dependent.

(Reed and Orr-Weaver, 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mr¹

(Braun et al., 2021, Whitfield et al., 2013, Chen et al., 2009, Christensen et al., 2009.2.28, Christensen et al., 2008.10.20, Pesin and Orr-Weaver, 2007, Tanaka-Matakatsu et al., 2007, Sugimura and Lilly, 2006)

Name Synonyms

morula1

(Narbonne-Reveau et al., 2008)

Secondary FlyBase IDs

References (14)

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