FlyBase Allele Report: Dmel\eff[8]

General Information

Symbol

Dmel\eff⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0028176

Feature type

allele

Associated gene

Dmel\eff

Associated Insertion(s)

P{PZ}eff⁸

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Cenci et al., 1997, Castrillon et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{PZ}eff⁸

Cytology

Description

The P{PZ} insertion is 432bp upstream of the putative ATG start codon.

(Fernandez-Funez et al., 2000)

Insertion of P{PZ} between bp 317and bp 318 (nucleotide positions numbered with respect to male germline 2.1kb transcript).

(Cenci et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:14,740,834..14,740,834 [-]

(Fernandez-Funez et al., 2000, Cenci et al., 1997, BDGP Project Members, 1994-1999, Castrillon et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^30Q.UAS

(Fernandez-Funez et al., 2000)

modeled by Hsap\ATXN1^82Q.UAS

(Fernandez-Funez et al., 2000)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber & cell

(Lilly et al., 2000)

egg chamber & mitotic cell cycle

(Lilly et al., 2000)

female germline stem cell (with eff^mer1)

(Chen et al., 2009)

female germline stem cell (with eff^mer4)

(Chen et al., 2009)

female germline stem cell (with eff^s1782)

(Chen et al., 2009)

female germline stem cell | germline clone

(Chen et al., 2009)

germarium

(Chen et al., 2009)

mitosis & chromosome, telomeric region

(Cenci et al., 1997)

neuroblast | somatic clone

(Watts et al., 2003)

nurse cell & nucleus

(Lilly and Spradling, 1996)

nurse cell fusome

(Lilly et al., 2000)

testis

(Castrillon et al., 1993)

Detailed Description

Statement

Reference

Approximately 30% of mutant ovarioles have either empty or abnormal germaria in 7 day old homozygous females. This germ cell defect becomes more severe with age.

The average number of germline stem cells per germarium is reduced in 14 day old eff⁸/eff^s1782 females compared to controls.

The average number of germline stem cells per germarium is reduced in 7 and 14 day old eff⁸/eff^mer4 and eff⁸/eff^mer1 females compared to controls.

Homozygous eff⁸ germline stem cell clones are lost more quickly from ovaries compared to control clones.

(Chen et al., 2009)

No defects are seen in axon pruning during metamorphosis, though a moderate proliferation defect is seen when somatic clones are made in the neuroblast.

(Watts et al., 2003)

About 22% of egg chambers from eff⁸/eff^RK1859 females, and 5% of egg chambers from eff⁸ homozygote mothers contain 32-cell cysts. Large fusomes with an increased number of branches relative to wild-type are also seen.

(Lilly et al., 2000)

Mitotic but not male meiotic telomere behaviour is abnormal in homozygotes. eff⁸/eff^Δ73A and eff⁸/eff^Δ112 transheterozygotes show abnormal telomere behaviour during both mitosis and male meiosis.

(Cenci et al., 1997)

Nurse cell chromocenters are increased dramatically in size.

(Lilly and Spradling, 1996)

Elongated spermatid nuclei are dispersed. Males are semi-sterile.

(Castrillon et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

eff^mer1/eff⁸ has female germline stem cell phenotype, enhanceable by mr¹/mr²

(Chen et al., 2009)

eff^mer1/eff⁸ has female germline stem cell phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/CycA^UASp.cCa

(Chen et al., 2009)

Suppressed by

Statement

Reference

eff⁸ has egg chamber phenotype, suppressible by CycA[+]/CycA⁰¹⁵⁴⁵

(Lilly et al., 2000)

eff⁸ has egg chamber phenotype, suppressible by Df(2R)59AB

(Lilly et al., 2000)

NOT suppressed by

Statement

Reference

eff⁸ has egg chamber phenotype, non-suppressible by Df(2L)r10

(Lilly et al., 2000)

Enhancer of

Statement

Reference

eff⁸ is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

eff⁸ is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

eff⁸ is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

eff⁸ is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

mr¹/mr² enhances the loss of germline stem cells that is seen in eff⁸/eff^mer1 females, such that the rate of germline stem cell loss is more rapid in the double mutant females.

Expression of CycA^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} strongly enhances the germline stem cell phenotype in eff⁸/eff^mer1 females, such that germline stem cell loss is more rapid and more severe in the double mutant females.

(Chen et al., 2009)

The 32 cell cyst phenotype is suppressible by adding heterozygous CycA⁰¹⁵⁴⁵, reducing the proportion of abnormal cysts seen to less than 6%. The addition of Df(2R)59AB also suppresses the phenotype, lowering the number of abnormal cysts seen by threefold. Df(2L)r10 however has no discernible effect on this phenotype.

(Lilly et al., 2000)

The extent of ectopic wing vein formation and roughening of the eye found in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS flies is enhanced in a eff⁸ mutant background.

(Robertson et al., 2000)

Xenogenetic Interactions

Statement

Reference

eff⁸ enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

eff⁸ enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Complementation and Rescue Data

Fails to complement

eff^mer4

(Fauvarque et al., 2001)

eff^mer1

(Fauvarque et al., 2001)

eff⁰¹⁴⁶²

(BDGP Project Members, 1994-1999)

eff¹³

(BDGP Project Members, 1994-1999)

eff¹²

(BDGP Project Members, 1994-1999)

eff¹¹

(BDGP Project Members, 1994-1999)

eff¹⁰

(BDGP Project Members, 1994-1999)

eff⁹

(BDGP Project Members, 1994-1999)

Rescued by

eff^mer1/eff⁸ is rescued by Scer\GAL4^{VP16.nanos.UTR}/eff^UASp.cCa

(Chen et al., 2009)

Not rescued by

eff^mer1/eff⁸ is not rescued by Scer\GAL4^C587/eff^UASp.cCa

(Chen et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11779

ry⁵⁰⁶ P{PZ}eff⁸/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)neo42⁰²⁴⁰⁴. Complements: l(3)03719⁰³⁷¹⁹. Complements: btsz¹⁰⁴¹⁸.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

UbcD1¹²⁹⁵

(Lilly et al., 2000)

UbcD1^eff-8

(Fauvarque et al., 2001)

UbcD1^effete8

(Cenci et al., 1997)

eff⁰¹²⁹⁵

eff⁸

(Ji et al., 2012, Wang and Pai, 2011, Chen et al., 2009, Branco et al., 2008, Rathke et al., 2007, Silva et al., 2007, Kuo et al., 2006, Robertson et al., 2000)

ms(3)01295

(Beaton, 1999.12.12)

ms(3)01295⁰¹²⁹⁵

Name Synonyms

Secondary FlyBase IDs

References (19)

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