FlyBase Allele Report: Dmel\awd[K]

General Information

Symbol

Dmel\awd^K

Species

D. melanogaster

Name

Killer of prune

FlyBase ID

FBal0000811

Feature type

allele

Associated gene

Dmel\awd

Associated Insertion(s)

Carried in Construct

Also Known As

awd^K-pn, awd^Kpn, Kpn, K-pn

Key Links

Genomic Maps

JBrowse

Allele class

neomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

neomorphic allele - genetic evidence

(Zinyk et al., 1993)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: P?S.

(Timmons and Shearn, 1995)

Amino acid replacement: S97P.

(Timmons et al., 1995)

Amino acid replacement: P97S.

(Lascu et al., 1992)

The levels of awd transcript are normal in (1) pn, (2) awd^K/+, and (3) pn; awd^K/+ flies; however, only the first two genotypes accumulate normal levels of the awd polypeptide, the last having little or none, despite the presence of one copy of awd⁺.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,745,431..31,745,431 [-]

Nucleotide change:

C31745431T

Amino acid change:

P112S | awd-PC; P112S | awd-PD; P97S | awd-PE

Reported amino acid change:

P97S

(Lascu et al., 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurofibromatosis

CEA with pn^MS2

(Hackstein, 1992)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Timmons et al., 1995, Sturtevant, 1955)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous ovaries occasionally show degenerating egg chambers at early stages (5% of ovarioles), but no significant epithelial defects are seen in the follicle cells.

(Woolworth et al., 2009)

Homozygous larvae develop normally.

(Timmons et al., 1995)

A small number of pn; awd^K larvae reach second instar. A maternal effect can shift the phenocritical period for one larval stage. This effect is temperature sensitive: low temperature, more larvae reach third instar. The perineurium, glia and lymph gland are the main cellular targets of the pn; awd^K interaction.

(Hackstein, 1992)

No phenotypic effects, either when homozygous or heterozygous.

(Sturtevant, 1955)

No phenotypic effects, either when homozygous or heterozygous; however, it is a dominant conditional lethal, which is lethal in combination with pn, i.e., pn/Y; awd^K/+ males or pn/pn; awd^K/+ females. awd^K does not interact with any other eye-color mutant. Interacts lethally with all standard alleles of pn tested; however, pn^ts-ek insensitive to awd^K at permissive temperatures, whereas nine other temperature-sensitive alleles of pn are insensitive under all conditions. Lethal phase of pn; awd^K genotypes begins in early second larval instar and lasts until after the time that normal larvae pupate. Using pn^ts-ek, the temperature-sensitive period of the pn component of the lethal interaction determined to begin in second instar and last until eclosion. pn; awd^K sons of pn mothers appear to die earlier than those of pn/+ mothers, suggesting a maternal effect of pn⁺, which is revealed in the presence of awd^K (FBrf0023024).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

awd^K/awd^K is a non-enhancer of paralytic | heat sensitive phenotype of shi^CK2

(Krishnan et al., 2001)

Suppressor of

Statement

Reference

awd^K is a suppressor of lethal phenotype of hop^Tum

(Zinyk et al., 1993)

Other

Statement

Reference

awd^K/awd^KRS6, pn¹ has lethal phenotype

(Krishnan et al., 2001)

awd^K/awd^MSM95, pn¹ has lethal phenotype

(Krishnan et al., 2001)

awd^K, pn¹ has lethal phenotype

(Krishnan et al., 2001, Timmons and Shearn, 1996)

awd^K, pn³⁸ has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn^pw2 has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn^A has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn^12C has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn^18a has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn² has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn³ has lethal phenotype

(Timmons and Shearn, 1996)

awd^K, pn^77C33 has lethal phenotype

(Timmons and Shearn, 1996, Timmons et al., 1995)

awd^K, pn^unspecified has lethal | dominant phenotype

(Timmons and Shearn, 1995)

awd^K, pn^77C33 has lethal | dominant phenotype

(Timmons et al., 1995)

awd^K, pn² has lethal | second instar larval stage phenotype

(Sturtevant, 1955)

awd^K, pn³ has lethal | second instar larval stage phenotype

(Sturtevant, 1955)

awd^K, pn¹ has lethal | second instar larval stage phenotype

(Sturtevant, 1955)

awd^K, pn^unspecified has lethal | dominant | larval stage phenotype

Phenotype Manifest In

Suppressor of

Statement

Reference

awd^K is a suppressor of melanotic mass phenotype of hop^Tum

(Zinyk et al., 1993)

awd^K is a suppressor of hemocyte phenotype of hop^Tum

(Zinyk et al., 1993)

Additional Comments

Genetic Interactions

Statement

Reference

Lethality of awd^K/+, pn^77C33 double mutants is not due to an inadequate level of awd NDP kinase activity.

(Timmons et al., 1995)

Rescues lethality of hop^Tum, alleviating premature formation of circulating haemocytes and melanotic mass formation in temperature- dependent fashion.

(Zinyk et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

awd^K is rescued by awd^+m.cPa

(Provost et al., 2006)

Comments

Allelism of awd^K demonstrated by two observations. First, induced revertants of awd^K, as recovered on the basis of their failure to interact lethally with pn, are lethal in all pairwise combinations (FBrf0020566) and in combination with awd^b3. Second transformants carrying awd^K DNA are able to rescue awd^b3 lethality and are able to kill pn in the presence of homozygous awd⁺ (FBrf0051397).

Images (0)

Mutant

Wild-type

Stocks (9)

Bloomington

461

ca¹ awd^K

42689

mu2^c ca¹ awd^K

1296

C(1)M3, y¹/Dp(1;Y)y⁺; P{wA^R}tsh^4-3; awd^K

1018

C(1)M3, y¹/Dp(1;Y)y⁺; P{A92}tshⁱ⁷¹; ca¹ awd^K

Kyoto

101584

ca¹ awd^K

130387

CyO/Pu²; In(3LR)HR33/T(Y;3)B116, Bar^S: ca¹ awd^K

106268

C(1)M3, y¹/Dp(1;Y)y⁺; P{wA^R}tsh^4-3; awd^K

105884

S¹/In(2L)Cy, In(2R)Cy, Duox^Cy E(S)¹ NimA^j-1; awd^K

107136

esc²¹ Adc^b-1 cn¹/In(2LR)Gla, wg^Gla-1; ca¹ awd^K

Notes on Origin

Discoverer

Sturtevant, Jan. 1954.

(Sturtevant, 1955)

Comments

Fate mapping in pn/pn/+; awd^K/+ gynandromorphs places the focus of the lethal interaction in the midventral part of the blastoderm, not a focus of pteridine biosynthesis. awd^K eye discs transplanted into pn hosts develop autonomously as do the reciprocal transplants (FBrf0011893).

P-element insertion carrying awd^K construct rescued the lethality of awd^b3 and an insertion into the third chromosome was alone sufficient to cause an interaction with pn.

(Biggs et al., 1988)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

K-pn

(Lascu et al., 1992, Sturtevant, 1955)

Kpn

(Provost et al., 2006, Timmons et al., 1995)

awd^K-pn

(Woolworth et al., 2009, Dearolf, 1998, de la Rosa et al., 1995, Watson et al., 1994, Hackstein, 1992, Venkatesh and Teng, 1992, Teng et al., 1991, Biggs et al., 1988)

awd^K

awd^Kpn

(Provost et al., 2006, Krishnan et al., 2001, Timmons and Shearn, 1995, Timmons and Shearn, 1995)

Name Synonyms

Killer of prune

(Provost et al., 2006)

Secondary FlyBase IDs

References (22)

Report Sections

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