FlyBase Allele Report: Dmel\Pde4[ML]

General Information

Symbol

Dmel\Pde4^ML

Species

D. melanogaster

Name

FlyBase ID

FBal0002718

Feature type

allele

Associated gene

Dmel\Pde4

Associated Insertion(s)

Carried in Construct

Also Known As

dnc^ML

Key Links

Allele class

amorphic allele - genetic evidence

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Salz and Kiger, 1984)

hypomorphic allele - genetic evidence

(Levine et al., 1994)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1³

(Kanellopoulos et al., 2012)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage (with Pde4^dnc-1)

(Nagarkar-Jaiswal et al., 2015)

abnormal learning | recessive

(Dauwalder and Davis, 1995)

abnormal locomotor rhythm

(Williams et al., 2001)

abnormal sleep

(Hendricks et al., 2001)

abnormal starvation stress response | third instar larval stage

(Koon et al., 2011)

female sterile

(Dauwalder and Davis, 1993)

female sterile | recessive

(Dauwalder and Davis, 1995)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Koon et al., 2011)

embryonic/larval neuromuscular junction | third instar larval stage (with Pde4^dnc-M14)

(Koon et al., 2011)

multidendritic neuron | third instar larval stage

(Koon et al., 2011)

multidendritic neuron | third instar larval stage (with Pde4^dnc-M14)

(Koon et al., 2011)

Detailed Description

Statement

Reference

dnc¹/dnc^ML adults show a significant reduction in performance index in an aversive olfactory learning assay compared to control flies.

(Nagarkar-Jaiswal et al., 2015)

dnc^ML homozygous and dnc^M14/dnc^ML heterozygous mutants exhibit an increase in the number of naturally occurring synaptopods at the type II endings. This increase is eliminated in a Dp(1;2)51b background.

(Koon et al., 2011)

Mutant flies show a significant reduction in the number of rest hours per 24 hours compared to controls.

(Hendricks et al., 2001)

Some dnc^ML flies are arrhythmic with respect to locomotor activity (only 63.16% are rhythmic).

(Williams et al., 2001)

Flies have a low learning score (0.15) in an olfactory classical conditioning paradigm. Homozygous females are sterile. Fertility is restored to approximately 50% if dnc^hs.PD is expressed using heat shock.

(Dauwalder and Davis, 1995)

The point of maximal phase delay of the light induced phase response curve (PRC) differs significantly from that of wild type and the circadian period is significantly shorter.

(Levine et al., 1994)

Flies transformed with the P{dnc^hs.P} or P{rat\dnc^hs.P} construct display increased learning scores. Reduced learning and memory.

(Dauwalder and Davis, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pde4^ML has abnormal learning | recessive phenotype, suppressible by Rat\RD1^hs.PD

(Dauwalder and Davis, 1995)

Suppressor of

Statement

Reference

Pde4^ML/dnc[+] is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Pde4^ML/dnc[+] is a suppressor of abnormal learning | dominant | adult stage phenotype of Fmr1³

(Kanellopoulos et al., 2012)

Pde4^ML is a suppressor of abnormal size phenotype of Nf1^unspecified

(Williams et al., 2001)

NOT Suppressor of

Statement

Reference

Pde4^ML is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1^P1

(Williams et al., 2001)

Pde4^ML is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1^P2

(Williams et al., 2001)

Pde4^ML is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1^unspecified

(Williams et al., 2001)

Other

Statement

Reference

Nf1^unspecified, Pde4^ML has partially lethal phenotype

(Williams et al., 2001)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Trans-heterozygotes of Fmr1³ and dnc^ML do not exhibit learning deficits.

(Kanellopoulos et al., 2012)

The low learning score phenotype is completely rescued by Rat\RD1^hs.PD expressed using heat shock.

(Dauwalder and Davis, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pde4^ML/Pde4^dnc-M14 is rescued by Pde4^UAS.cCa/Scer\GAL4^Tdc2.PC

(Koon et al., 2011)

Pde4^ML is rescued by Pde4^UAS.cCa/Scer\GAL4^Tdc2.PC

(Koon et al., 2011)

Comments

Expression of dnc^Scer\UAS.cCa exclusively in octopaminergic neurons under the control of Scer\GAL4^Tdc2.PC rescues the increase in synaptopods at neuromuscular junction type II endings seen in dnc^ML homozygous and dnc^M14/dnc^ML heterozygous mutants.

(Koon et al., 2011)

The low learning score phenotype is partially rescued by dnc^hs.PD expressed using heat shock. Fertility is restored to approximately 50% if dnc^hs.PD is expressed using heat shock.

(Dauwalder and Davis, 1995)

A partial rescue is seen after induction of dnc^hs.P. A significant increase in the initial learning score is seen. Heat shock pulses four days after hatching partly rescues female fertility.

(Dauwalder and Davis, 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Enzyme activity is 3% of wild-type.

(Salz and Kiger, 1984)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pde4^ML

dnc^ML

(Dannhäuser et al., 2020, Nagarkar-Jaiswal et al., 2015, Walker et al., 2013, Kanellopoulos et al., 2012, Koon et al., 2011, Hendricks et al., 2001)

dunce^ML

(Dannhäuser et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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