XX dsx¹/dsx^D animals do not show an increase in midline crossing by foreleg gustatory receptor neuron (GRN) axons compared to the extremely low level of midline crossing seen in control XX females.

P1 neurons are present in both XX and XY dsx^D/Df(3R)dsx15 animals (these neurons are normally only found in male brains in the wild type).

XX germ cells proliferate in dsx^D/dsx¹ mutant embryos (in contrast to wild type, where germ cells do not proliferate in XX embryos).

Female dsx^D/dsx¹ stage 15 gonads exhibit a completely masculinized phenotype, in which male-specific somatic gonadal precursors are clearly present.

X/X tra¹/tra¹ tra^hs.PBa and X/Y tra¹/tra¹ tra^hs.PBa flies have a female body and pheromone pattern and display indiscriminate sexual behaviour. By introducing dsx^D mutants now have a male body and male pheromone pattern, these flies are unattractive to males but will still court both sexes.

XX dsf¹ dsx^D double hemizygotes have wild-type ventral abdominal muscle innervation phenotype, while XY dsf⁺ tra^- dsf¹ dsx^D double hemizygotes have a dsf¹ ventral abdominal muscle innervation phenotype, indicating that dsf acts independently of dsx.

XX D.teissieri-D.melanogaster hybrid flies carrying one copy of dsx^D have an almost completely normal set of male genital structures. Vaginal plates are almost always absent and the female eighth tergite is incomplete or missing. The penis apparatus is more normal in terms of the structures present and their differentiation in XX D.teissieri-D.melanogaster dsx^D/+ hybrid flies than in XX D.melanogaster dsx^D/+ flies. The seventh tergite and sternite are present in XX D.melanogaster dsx^D/+ flies. The seventh sternite is absent and the seventh tergite is rudimentary in XX D.teissieri-D.melanogaster dsx^D/+ hybrid flies.

dsx^D/+ and dsx^D/dsx^3-A135 adults are hypersensitive to paraquat.

dsx^D is unable to suppress the sex comb defect seen in homozygous male U2af38⁰⁶⁷⁵¹ flies.

XX dsx^D/+, dsx^D/Df(3R)dsx15 and ix¹/ix¹; dsx^D/+ flies do not show any male-specific courtship when paired with mature virgin females. 88% of XY dsx^D/Df(3R)dsx15 flies, 100% of XY dsx^D/+ flies and 50% of XY ix¹/ix¹; dsx^D/+ flies show male-specific courtship when paired with mature virgin females.

Sex specific neuroblasts fail to undergo any postembryonic divisions in male or female larval nervous systems.

Heterozygous flies are intersex, having both male and female reproductive organs.

vir^ts/vir^ts dsx^D/+ XX flies have a dsx phenotype at 25^oC, and are strongly masculinised intersexes or almost pseudomales with poorly developed gonads at 29^oC.

Chromosomal females (XX) mutant for dsx are transformed into "pseudomales. They are sterile even in the presence of a Y chromosome. The gonads form testes which are generally non-gametogenic, containing degenerated germ cells and debris or gonial cells whose sex cannot be determined. A variable proportion are gametogenic, containing either oogenic or spermatogenic stages of germ cell development (never both in the same gonad). In these cases oogenesis is arrested anywhere between stages S3 and S10A, and spermatogenesis is frequently arrested at the spermatocyte stage, although some later stages of spermatogenic differentiation are seen.

Only affects XX flies: the terminalia of XX heterozygous flies carry abnormal male and female elements, with the female genitalia anterior to the male genitalia. The male genitalia are well developed, but the penis apparatus is reduced, the hypandrium is mostly absent and many bristles are abnormal. The female vaginal plate is always present, but is reduced in size with abnormal bristles. There is often a mass of yellow, chitinised material between the vaginal plates, which is a secondary rudimentary penis apparatus. The anal plates have a sexually intermediate shape, and a bristle pattern which is a mosaic of male, female and intermediate bristles. The basitarsus of the foreleg carries a sex comb whose position and shape are intermediate between male and female. XX hemizygotes are sterile pseudomales.

Flies are intersex, having two somewhat abnormal and incomplete sets of genitalia.

Effects only XX flies. Homozygous XX flies have rudimentary gonads, occasionally with some oocytes and eggs. dsx^D/dsx¹ flies are transformed into somatically male-like animals.

Female intersexuals.

dominant; affects XX only

dsx^D has abnormal sex-determination | dominant | female phenotype, enhanceable by tra[+]/tra^unspecified

dsx^D has abnormal sex-determination | dominant | female phenotype, enhanceable by ix^unspecified/ix[+]

dsx^D has abnormal sex-determination | dominant | female phenotype, enhanceable by tra2[+]/tra2^unspecified

Df(3R)fru^sat15, dsx^D has abnormal sex-determination | adult stage phenotype

dsx^D, fru^ΔB has abnormal sex-determination | adult stage phenotype

Df(3R)fru^sat15, dsx^D has gonad | adult stage phenotype

dsx^D, fru^ΔB has gonad | adult stage phenotype