FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\fs(1)YbM104-3
Open Close
General Information
Symbol
Dmel\fs(1)YbM104-3
Species
D. melanogaster
Name
FlyBase ID
FBal0004320
Feature type
allele
Associated gene
Dmel\fs(1)Yb
Associated Insertion(s)
Carried in Construct
Also Known As
Yb6
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Johnson et al., 1995)
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Nucleotide substitution: G2573A. Amino acid replacement: C?Y.

(King and Lin, 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:2,723,037..2,723,037 [+]
Nucleotide change:

G2723037A

Reported nucleotide change:

G2573A

Amino acid change:

C804Y | fs(1)Yb-PA

Reported amino acid change:

C?Y

(King and Lin, 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous fs(1)YbM104-3 ovarioles contain but 0-2 egg chambers but lack germaria due to a failure in germline stem cell maintenance.

      (King et al., 2001)

      15% of ovarioles of newly eclosed homozygous females have 1-3 egg chambers (instead of 5 as in wild type) and no germline cells in the germarium. 43% have 1-3 egg chambers and undifferentiated germline cells in the germarium. 2% have 1-3 egg chambers and differentiated germline cells in the germarium. 15% have no egg chambers and undifferentiated germline cells in the germarium. 26% have no egg chambers and differentiated germline cells in the germarium. 12% of ovarioles of newly eclosed fs(1)YbM104-3/Df(1)w258-45 females have 1-3 egg chambers (instead of 5 as in wild type) and no germline cells in the germarium. 19% have 1-3 egg chambers and undifferentiated germline cells in the germarium. 48% have 1-3 egg chambers and differentiated germline cells in the germarium. 7% have no egg chambers and undifferentiated germline cells in the germarium. 15% have no egg chambers and differentiated germline cells in the germarium. Germline clonal analysis indicates that fs(1)Yb is not required in the germline for fertility. Homozygous germline clones have normal ovarioles containing a normal germarium and can produce a number of mature eggs.

      (King and Lin, 1999)

      Follicle cells fail to invaginate between the developing egg cysts in the germarium, leading to a gap in the follicle cell layers between stage 1 egg chambers and the succeeding cyst. Composite chambers develop. Compound chambers in older females become increasingly disorganized, many containing dying germ cells with small pycnotic nuclei. Stalk cells appear to be missing, and egg chambers cluster together. Mutant eggs are smaller than wild type and have a dumpy phenotype because the dorsal surface is rounded. Dorsal appendages are often shortened and abnormally shaped.

      (Johnson et al., 1995)

      Ovaries have abnormally shaped yolks.

      (Mohler and Carroll, 1984)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      fs(1)YbM104-3 has germarium phenotype, suppressible by ptc16

      (King et al., 2001)

      fs(1)YbM104-3 has germline stem cell phenotype, suppressible by ptc16

      (King et al., 2001)

      fs(1)YbM104-3 has egg chamber phenotype, suppressible by ptc16

      (King et al., 2001)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      fs(1)YbM104-3 homozygous ovarioles that contain ptc16 clones contain functional germaria and an average of 4.6 post-germarial egg chambers. Some ovarioles contain up to seven egg chambers. However these germaria are somewhat rudimentary and comparable in size and morphology to old wild-type germaria with only one remaining germline stem cell.

      (King et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      14-926
      (Mohler and Carroll, 1984)
      Yb6
      (Szakmary et al., 2009, King et al., 2001, King and Lin, 1999)
      fs(1)M1043
      fs(1)Yb6
      (Johnson et al., 1995)
      fs(1)YbM104-3
      Name Synonyms
      Secondary FlyBase IDs
        References (5)