FlyBase Allele Report: Dmel\grk[1]

General Information

Symbol

Dmel\grk¹

Species

D. melanogaster

Name

FlyBase ID

FBal0005209

Feature type

allele

Associated gene

Dmel\grk

Associated Insertion(s)

Carried in Construct

Also Known As

grk^DC, grk^DC29

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neuman-Silberberg and Schupbach, 1996, Neuman-Silberberg and Schupbach, 1993, Schupbach and Wieschaus, 1991, Schupbach, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: A245V. Nucleotide substitution: C2424T.

(Thio et al., 2000)

Amino acid replacement: A245V.

(Queenan et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,431,693..8,431,693 [-]

Nucleotide change:

C8431693T

Reported nucleotide change:

C2424T

Amino acid change:

A246V | grk-PA

Reported amino acid change:

A245V

(Thio et al., 2000, Queenan et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Schupbach and Wieschaus, 1991)

female sterile | recessive

(Neuman-Silberberg and Schupbach, 1993)

Phenotype Manifest In

border follicle cell (with grk^2B6)

(Duchek and Rorth, 2001)

dorsal appendage

(Schupbach, 1987)

egg operculum

(Schupbach, 1987)

embryo | ventral

(Schupbach, 1987)

microtubule & oocyte

(Clegg et al., 1997)

oocyte & microtubule (with grk^2B6)

(Januschke et al., 2002)

ovary (with grk^2B6)

(Januschke et al., 2002, Duchek and Rorth, 2001)

ventral furrow

(Schupbach, 1987)

Detailed Description

Statement

Reference

In grk^2B6/grk¹ oocytes the anterior-posterior gradient of microtubule concentration seen in wild-type is abolished. However, concentration of microtubules around the mis-localized nucleus persists.

(Januschke et al., 2002)

Ovaries from grk¹/grk^2B6 mutant females show a range of defects. In mildly affected egg chambers where the germinal vesicle has moved anterior and dorsal, border cells complete posterior migration but fail to migrate dorsally.

(Duchek and Rorth, 2001)

Microtubule cytoskeleton in grk^2B6/grk¹ oocytes is defective due to premature streaming of the microtubules.

(Clegg et al., 1997)

grk¹/grk² heterozygous females display fused egg chambers and gaps in the follicular epithelium that uncover the nurse cells. Eggs derived from these females exhibit strong ventralised phenotype.

(Goode et al., 1996)

Reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle at both ends of eggs, embryos ventralized.

(Schupbach and Wieschaus, 1991)

An enlarged mass of mesodermal cells invaginates on the ventral side of the embryo, the enlarged mesoderm is often organised into two ventral furrows. Organisation is lost in later stages of development and a mass of mesodermal cells fills the ventral half of the embryo. Respiratory appendages are completely absent and only the ventral most portion of the operculum is present.

(Schupbach, 1987)

phenotype of eggs and embryos: intermediate and strong

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong grk allele.

(Schupbach, 1987)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

grk¹

grk^DC29

(Jordan et al., 2000, Thio et al., 2000, Larkin et al., 1999, Queenan et al., 1999, Clegg et al., 1997, Goode et al., 1996, Morimoto et al., 1996)

grk^DC9

(Bryant et al., 1999)

grk^DC

(Bokel et al., 2006, Januschke et al., 2006, Cui et al., 2005, Januschke et al., 2002, Schnorrer et al., 2002, Dequier et al., 2001, Duchek and Rorth, 2001, Morisato, 2001, Grammont et al., 2000, Schnorrer et al., 2000, Doerflinger et al., 1999, Peri et al., 1999, Neuman-Silberberg and Schupbach, 1996, Roth et al., 1995, Neuman-Silberberg and Schupbach, 1993, Schupbach, 1987)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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