microtubule & oocyte
Eggs derived from females expressing dppScer\UAS.cSa under the control of Scer\GAL4CY2 in a grk2B6/grkHF background have misshaped micropyles, show the loss of the collar that normally separates the operculum from the rest of the chorion and show a reduction in egg diameter.
Stage 12 grk3/grk2B6 ; cicfet-U6/cicfet-T6 double mutant egg chambers lack dorsal appendages (characteristic of loss of grk), but retain a pronounced collar of appendage material, indicating that loss of grk cannot suppress the cic phenotype.
Heterozygosity for grk2B6 enhances the penetrance of dorsal appendage loss or fusion in eggs laid by Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa females, from 5% to 90%. This phenotype still shows over 90% penetrance in eggs laid by Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa; grk2B6/+; vasαTub67C.T:Avic\GFP flies.
The presence of one or two copies of Dwil\GK18382+ta in grk2B6/grk2E12 mutant D. melanogaster flies results in four distinct eggshell phenotypes. Most eggshells, 69% and 78% from one and two copies of Dwil\GK18382+ta, respectively, are wild-type or wild-type with an enlarger area posterior to the base of the dorsal appendages, similar to wild-type flies expressing Dwil\GK18382+ta. A dorsal ridge-like morphology is found in 10% and 1% of the eggshells from one and two copies of Dwil\GK18382+ta, respectively.