Stage 12 grk3/grk2B6 mutant egg chambers lack dorsal appendages.
Mutant females produce eggs with strongly ventralised chorions.
About half of heterozygotes show a weak dorsal appendage phenotype where the two appendages are closer together than in wild-type. Stronger dorsal appendage phenotypes are occasionally seen.
grk6/grk3 females produce weakly ventralized egg chambers. Eggs laid have lost their dorsalmost fates and have a single medially fused dorsal appendage.
Eggs derived from fs(1)K10unspecified; grk3/+ females show a broad spectrum of phenotypes ranging from completely dorsalised to completely ventralised.
Dorsal appendages are missing and there is no evidence of dorso-ventral polarity.
Homozygous eggs completely lack dorsal appendages.
Homozygous females display fused egg chambers and gaps in the follicular epithelium, females lay completely ventralised eggs. Eggs derived from heterozygous mothers show slight haploinsufficiency for dorsal-ventral patterning of the follicular epithelium. Eggs derived from brnl.6P6/brn+; grk3/grk+ females show an intermediate to strong fused dorsal appendage phenotype that is indistinguishable from eggs laid by brn females.
Ventralised egg chambers. Egg chambers become elongated in shape, dorsal appendages fuse and shrink and the number of main body follicle cells increases.
Almost no eggs laid by heterozygous females display a fused dorsal appendage phenotype.
Posterior follicle cells show an abnormal shape and behaviour.
Weak and intermediate ventralised embryos. orbF343 and orbdec interact genetically with grk, double heterozygote embryos display fused dorsal appendages.
Females heterozygous for grk3 produce 80-90% wild type eggs.
grk3/grk6 causes strong ventralization of embryos: 71% of eggs have reduced and 22% of eggs have no dorsal appendages. 2% of the eggs contain embryos.
Eggs laid by homozygous females have no dorsal appendages. The egg chamber in homozygous females is elongated and the anteriorly located follicle cells fail to migrate, resulting in a thin and regular sheet of follicle cells around the oocyte. grk3 homozygous females also heterozygous or homozygous for fs(1)K1013 or fs(1)K101 produce eggs with a ventralised phenotype. fs(1)K1013 homozygous females also heterozygous for grk3 produce eggs with a range of phenotypes, from that of fs(1)K1013 homozygotes to almost wild-type. Approximately 70% of the eggs produce an intermediate phenotype with two dorsal appendages that are located at the very anterior end of the egg. The embryos derived from these eggs also show a range of phenotypes, from a dorsalised to an almost wild-type cuticle. Rescue always occurs first at the posterior end of the embryo, with the cephalic structures being the last to differentiate normally and often being everted.
Embryos from double mutants with sqdix43 are weakly ventralised.
Follicular epithelium of mutant egg chambers has holes.
Reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle at both ends of eggs, embryos ventralized.
An enlarged mass of mesodermal cells invaginates on the ventral side of the embryo, the enlarged mesoderm is often organised into two ventral furrows. Organisation is lost in later stages of development and a mass of mesodermal cells fills the ventral half of the embryo. Respiratory appendages are completely absent and only the ventral most portion of the operculum is present.
phenotype of eggs and embryos: intermediate and strong