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General Information
Symbol
Dmel\grk3
Species
D. melanogaster
Name
FlyBase ID
FBal0257023
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
grkHK, grkHK36
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A 432bp insertion homologous to a fragment of the roo element is inserted at bp2336.

Insertion components
roo{}grk3
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Stage 12 grk3/grk2B6 mutant egg chambers lack dorsal appendages.

Mutant females produce eggs with strongly ventralised chorions.

About half of heterozygotes show a weak dorsal appendage phenotype where the two appendages are closer together than in wild-type. Stronger dorsal appendage phenotypes are occasionally seen.

grk6/grk3 females produce weakly ventralized egg chambers. Eggs laid have lost their dorsalmost fates and have a single medially fused dorsal appendage.

Eggs derived from fs(1)K10unspecified; grk3/+ females show a broad spectrum of phenotypes ranging from completely dorsalised to completely ventralised.

Dorsal appendages are missing and there is no evidence of dorso-ventral polarity.

Homozygous eggs completely lack dorsal appendages.

Homozygous females display fused egg chambers and gaps in the follicular epithelium, females lay completely ventralised eggs. Eggs derived from heterozygous mothers show slight haploinsufficiency for dorsal-ventral patterning of the follicular epithelium. Eggs derived from brnl.6P6/brn+; grk3/grk+ females show an intermediate to strong fused dorsal appendage phenotype that is indistinguishable from eggs laid by brn females.

Ventralised egg chambers. Egg chambers become elongated in shape, dorsal appendages fuse and shrink and the number of main body follicle cells increases.

Almost no eggs laid by heterozygous females display a fused dorsal appendage phenotype.

Posterior follicle cells show an abnormal shape and behaviour.

Strong allele.

Weak and intermediate ventralised embryos. orbF343 and orbdec interact genetically with grk, double heterozygote embryos display fused dorsal appendages.

Females heterozygous for grk3 produce 80-90% wild type eggs.

grk3/grk6 causes strong ventralization of embryos: 71% of eggs have reduced and 22% of eggs have no dorsal appendages. 2% of the eggs contain embryos.

Eggs laid by homozygous females have no dorsal appendages. The egg chamber in homozygous females is elongated and the anteriorly located follicle cells fail to migrate, resulting in a thin and regular sheet of follicle cells around the oocyte. grk3 homozygous females also heterozygous or homozygous for fs(1)K1013 or fs(1)K101 produce eggs with a ventralised phenotype. fs(1)K1013 homozygous females also heterozygous for grk3 produce eggs with a range of phenotypes, from that of fs(1)K1013 homozygotes to almost wild-type. Approximately 70% of the eggs produce an intermediate phenotype with two dorsal appendages that are located at the very anterior end of the egg. The embryos derived from these eggs also show a range of phenotypes, from a dorsalised to an almost wild-type cuticle. Rescue always occurs first at the posterior end of the embryo, with the cephalic structures being the last to differentiate normally and often being everted.

Embryos from double mutants with sqdix43 are weakly ventralised.

Follicular epithelium of mutant egg chambers has holes.

Reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle at both ends of eggs, embryos ventralized.

An enlarged mass of mesodermal cells invaginates on the ventral side of the embryo, the enlarged mesoderm is often organised into two ventral furrows. Organisation is lost in later stages of development and a mass of mesodermal cells fills the ventral half of the embryo. Respiratory appendages are completely absent and only the ventral most portion of the operculum is present.

phenotype of eggs and embryos: intermediate and strong

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference

grk3, grk3, grk[+] is an enhancer of egg chorion phenotype of Ras85Dix12a

grk3, grk3, grk[+] is an enhancer of embryonic epidermis phenotype of cactE10

grk3/grk3 is an enhancer of egg chorion phenotype of Ras85Dix12a

NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference

grk3, grk3, grk2B6 is a non-suppressor of dorsal appendage phenotype of cicfet-U6/cicfet-T6

grk3, grk3, grk2B6 is a non-suppressor of egg phenotype of cicfet-U6/cicfet-T6

grk3, grk3, grk[+] is a non-suppressor of egg chorion phenotype of Egfr::kek1KEΔCG.UAS.GFP, Scer\GAL4CY2

Other
Additional Comments
Genetic Interactions
Statement
Reference

grk3/grk2B6 suppresses the over proliferation of posterior follicle cells in Dis3G5039/Dis32 egg chambers.

Stage 12 grk3/grk2B6 ; cicfet-U6/cicfet-T6 double mutant egg chambers lack dorsal appendages (characteristic of loss of grk), but retain a pronounced collar of appendage material, indicating that loss of grk cannot suppress the cic phenotype.

The dorsalised chorion phenotype caused by expression of Egfr::kek1KEΔCG.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4CY2 is not suppressed if the flies are also heterozygous for grk3.

S48-5/grk3 mutants show 3.14%+-2.58 misrotated ommatidia compared to 9.55%+-1.43 seen in S48-5 mutants alone.

Homozygous females that are also heterozygous for grk3 show an enhancement of the eggshell phenotype.

The addition of Lis-13.1.2 or Lis-1k11702 enhances the dorsal appendage phenotype seen in grk3/ flies.

fs(1)K101; grk3 double mutant females produce eggs with ventralised eggshells and embryos.

Eggs from most BicDr8 and grk3 transheterozygotes display a fused dorsal appendage phenotype.

Partly corrects the misorientation of the dorsoventral and anteroposterior axes relative to one another seen in fs(1)K10unspecified embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Strong grk allele.

Strong mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (63)