Isolation and characterization of Df(2L)BSC201
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC201 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05793 and PBac{WH}CG13088f01520. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P {XP}d05793/PBac{WH}CG13088f01520 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC201 from the segment of P {XP}d05793 to the left of its FRT site and the segment of PBac{WH}CG13088f01520 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P {XP}d05793 to be at Release 3 genomic coordinate 8408662 on chromosome arm 2L. The Gene Disruption project determined the insertion site of P{XP}d05793 to be at Release 3 genomic coordinate 8408789 on arm 2L. These insertion sites are predicted to lie in 29C3 on both the Release 3 and Release 4 maps. The insertion site of PBac{WH}CG13088f01520 is predicted to lie in 29D1 on the Release 4 map. Consequently, the cytological breakpoints of Df(2L)BSC201 are predicted to be 29C3;29D1. It failed to complement dGC13 and grk3.