FlyBase Allele Report: Dmel\Dis3[2]

General Information

Symbol

Dmel\Dis3²

Species

D. melanogaster

Name

FlyBase ID

FBal0319957

Feature type

allele

Associated gene

Dmel\Dis3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Snee et al., 2016)

Mutagen

ethyl methanesulfonate

(Snee et al., 2016)

Progenitor genotype

Cytology

Description

Amino acid replacement: W360term.

(Snee et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,334,687..24,334,687 [+]

Nucleotide change:

G24334687A

Reported nucleotide change:

G1255A

Amino acid change:

W360term | Dis3-PA; W360term | Dis3-PB

Reported amino acid change:

W360term

(Snee et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of multiple myeloma

CEA

(Snee et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome | third instar larval stage (with Dis3¹), with Dis3^S786E.UASp, Scer\GAL4^Tub.PU

(Snee et al., 2016)

follicle cell | increased number (with Dis3^G5039)

(Snee et al., 2016)

imaginal disc | third instar larval stage (with Dis3¹), with Dis3^S786E.UASp, Scer\GAL4^Tub.PU

(Snee et al., 2016)

larval brain | third instar larval stage (with Dis3¹), with Dis3^S786E.UASp, Scer\GAL4^Tub.PU

(Snee et al., 2016)

larval neuroblast | larval stage (with Dis3¹)

(Snee et al., 2016)

larval neuroblast | third instar larval stage (with Dis3¹), with Dis3^S786E.UASp, Scer\GAL4^Tub.PU

(Snee et al., 2016)

larval ventral nerve cord | third instar larval stage (with Dis3¹), with Dis3^S786E.UASp, Scer\GAL4^Tub.PU

(Snee et al., 2016)

mitotic spindle | larval stage (with Dis3¹)

(Snee et al., 2016)

mitotic spindle | third instar larval stage (with Dis3¹), with Dis3^S786E.UASp, Scer\GAL4^Tub.PU

(Snee et al., 2016)

wing disc | second instar larval stage | somatic clone

(Snee et al., 2016)

Detailed Description

Statement

Reference

Dis3²/Dis3² mutants survive at the second instar larval stage for an extended period and then die. Dis3²/Dis3² wing disc clones in second instar larvae have significantly fewer cells per clone compared to controls and mutant cells undergo only one cell division in a 48hr time window (compared to 4 in wild type cells).

Dis3¹/Dis3² third instar larvae with expression of Dis3^{S786E.Scer\UAS.P\T} driven by Scer\GAL4^tub.PU lack imaginal discs, have reduced brain and ventral nerve cord size, whereas other tissues that do not grow via mitotic cell cycles (salivary glands) are generally normal in size and morphology; larval neuroblasts have multipolar, disorganized or short spindles, 5% of mitotic neuroblasts display aneuploidy, 44% display over condensed chromosomes and more neuroblasts are mitotically active than controls.

Dis3^G5039/Dis3² flies are viable, largely infertile and morphologically normal (apart from small bristles and occasional crumpled wings); a large percentage of stage 5-6 egg chambers show over proliferation, with excess posterior follicle cells.

(Snee et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dis3²/Dis3^G5039 has increased cell number phenotype, suppressible by grk³/grk^2B6

(Snee et al., 2016)

NOT suppressed by

Statement

Reference

Dis3² has decreased cell growth rate | somatic clone | second instar larval stage phenotype, non-suppressible by BacA\p35^UAS.cUa/Scer\GAL4^Tub.PU

(Snee et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dis3²/Dis3^G5039 has follicle cell | increased number phenotype, suppressible by grk³/grk^2B6

(Snee et al., 2016)

NOT suppressed by

Statement

Reference

Dis3² has wing disc | somatic clone | second instar larval stage phenotype, non-suppressible by BacA\p35^UAS.cUa/Scer\GAL4^Tub.PU

(Snee et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

grk³/grk^2B6 suppresses the over proliferation of posterior follicle cells in Dis3^G5039/Dis3² egg chambers.

(Snee et al., 2016)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cUa driven by Scer\GAL4^tub.PU does not significantly increase the number of cells per clone in Dis3²/Dis3² second instar larval wing discs.

(Snee et al., 2016)

Complementation and Rescue Data

Rescued by

Dis3² is rescued by Dis3^UASp.WT/Scer\GAL4^Tub.PU

(Snee et al., 2016)

Dis3² is rescued by Dis3^S786A.UASp/Scer\GAL4^Tub.PU

(Snee et al., 2016)

Dis3²/Dis3^G5039 is rescued by Scer\GAL4^Act.PU/Dis3^G5039

(Snee et al., 2016)

Partially rescued by

Dis3¹/Dis3² is partially rescued by Dis3^S786E.UASp/Scer\GAL4^Tub.PU

(Snee et al., 2016)

Not rescued by

Dis3² is not rescued by Scer\GAL4^Tub.PU/Dis3^S786E.UASp

(Snee et al., 2016)

Comments

Expression of Dis3^{Scer\UAS.P\T.WT} or Dis3^{S786A.Scer\UAS.P\T} (but not Dis3^{S786E.Scer\UAS.P\T}) driven by Scer\GAL4^tub.PU rescues the number of cells per clone in Dis3²/Dis3² second instar larval wing discs and lethality of Dis3²/Dis3² mutants. Expression of Dis3^{S786E.Scer\UAS.P\T} driven by Scer\GAL4^tub.PU rescues larval development until late wandering third instar larval stage (though larvae take 7-12 extra days to reach this stage and then die shortly after) in Dis3²/Dis3² mutants.

Expression of Dis3^G5039 driven by Scer\GAL4^Act.PU rescues over proliferation of follicle cell phenotypes in Dis3^G5039/Dis3² egg chambers.

(Snee et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

83001

w¹¹¹⁸; P{FRT(w^hs)}2A P{neoFRT}82B Dis3²/TM6B, P{Ubi-GFP.S65T}PAD2, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dis3²

dis3²

(Snee et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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