FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Dis3S786E.UASp
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General Information
Symbol
Dmel\Dis3S786E.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0319963
Feature type
allele
Associated gene
Dmel\Dis3
Associated Insertion(s)
Carried in Construct
P{UASp-Dis3.S786E}
Key Links
Transgenic product class
missense_variant
(Snee et al., 2016)
polypeptide_post_translational_processing_variant
(Snee et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Snee et al., 2016)
polypeptide_post_translational_processing_variant
(Snee et al., 2016)
Mutagen
in vitro construct
(Snee et al., 2016)
Progenitor genotype
Carried in construct
P{UASp-Dis3.S786E}
Cytology
Description

UASp regulatory sequences drive expression of a phosphomimetic form Dis3 that carries the amino acid replacement S786E.

(Snee et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Dis3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Dis31/Dis32 third instar larvae with expression of Dis3S786E.Scer\UAS.P\T driven by Scer\GAL4tub.PU survive to the third instar larval stage but lack imaginal discs and have reduced brain and ventral nerve cord size, whereas other tissues that do not grow via mitotic cell cycles (salivary glands) are generally normal in size and morphology; larval neuroblasts have multipolar, disorganized or short spindles, 5% of mitotic neuroblasts display aneuploidy, 44% display over condensed chromosomes and more neuroblasts are mitotically active than controls.

      (Snee et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Dis3S786E.UASp/Scer\GAL4Tub.PU partially rescues Dis31/Dis32

      (Snee et al., 2016)
      Fails to rescue

      Scer\GAL4Tub.PU/Dis3S786E.UASp fails to rescue Dis32

      (Snee et al., 2016)
      Comments

      Expression of Dis3S786E.Scer\UAS.P\T driven by Scer\GAL4tub.PU fails to rescue the number of cells per clone in Dis32/Dis32 second instar larval wing discs. Expression of Dis3S786E.Scer\UAS.P\T driven by Scer\GAL4tub.PU rescues larval development until late wandering third instar larval stage (though larvae take 7-12 extra days to reach this stage and then die shortly after) in Dis31/Dis32 mutants.

      (Snee et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dis3S786E.Scer\UAS.P\T
      Dis3S786E.UASp
      Name Synonyms
      Secondary FlyBase IDs
        References (1)