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General Information
Symbol
Dmel\cicfet-U6
Species
D. melanogaster
Name
FlyBase ID
FBal0130456
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
cicfetU6
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C20296056T

Reported nucleotide change:

C?T

Amino acid change:

Q764term | cic-PA; Q764term | cic-PB; Q764term | cic-PC; Q1502term | cic-PD; Q1506term | cic-PG; Q612term | cic-PH

Comment:

Approximate location of amber mutation cicfet-U6 in cic based on FBrf0141486.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amber mutation in exon 4.

Amino acid replacement: ??term.

Nucleotide substitution: C3007T.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous cicfet-U6 intestinal stem cell somatic clones (generated by MARCM) are larger than control clones and contain more cells.

Number of mitotic cells per midgut is significantly increased in cicfet-U6/cicfet-E11 transheterozygous adults.

Eggs derived from egg chambers containing complete homozygous follicle cell clones have a dorsalised eggshell.

Eggs from mosaic homozygous cicfet-U6 females exhibit ventral and lateral foci of ectopic appendage-like materail. Analysis of homozygous cicfet-U6 follicle cell clones confirms that these foci are associated with ventral and lateral clones of cicfet-U6 mutant clones. This ectopic appendage material is restricted to the anterior half of the eggshell, while posterior clones exhibit no detectable phenotype. Interestingly, ectopic appendage material at the extreme anterior of the eggshell often appears as appendage-like projections, suggesting that only cicfet-U6 mutant follicle cells in the corresponding region of the columnar follicular epithelium can form an appendage-producing epithelial tube.

With the exception of those at the dorsal midline, cicfet-U6 mutant follicle cell clones undergo apical constriction reminiscent of that observed in the endogenous appendage-producing populations. In addition, the follicle cell nuclei appear more closely packed in both the endogenous appendage roof primordia and in regions of anterior cicfet-U6 mutant cells, presumably as a consequence of this apical constriction.

Approximately 12.9% of cicfet-E11/cicfet-U6 females exhibit a weak eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally with a trace of ventral collar material. The dorsalmost region remains free of appendage material.

Approximately 19.3% of cicfet-U6/cicfet-E11 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 30.0% of cicfet-U6/cicfet-E11 exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 14.1% of cicfet-U6/cicBA53 exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 85.9% of cicfet-U6/cicBA53 exhibit a severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.

Approximately 32.8% of cicfet-U6/cicfet-T6 females exhibit a weak-moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 20.9% of cicfet-U6/cicfet-T6 exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 1.7% of cicfet-U6/cicfet-T6 exhibit a severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.

Eggs derived from cicfet-U6/cicfet-E11 females are dorsalised. The eggs are shorter than normal and have broader dorsal appendages that are shifted laterally, with appendage base material encircling the entire anterior circumference of the egg. Embryos derived from cicfet-U6/cicfet-E11 females fail to hatch and are dorsalised (the secreted cuticle consists entirely of dorsal epidermis). cicfet-U6/cicfet-E11 adults have smaller wings than normal, with vein thickening and blistering. 22% of eggs produced by females with homozygous germline clones are dorsalised (78% are wild type), but 100% of the embryos derived from these eggs fail to hatch (no cellularisation is seen in these embryos). 34% of eggs derived from females carrying homozygous follicle cell clones are dorsalised (66% are wild type). 54% of the embryos derived from these females fail to hatch (of these, 10% are locally dorsalised along the anterior-posterior axis and 1% are completely dorsalised), and 46% hatch. Dorsal or lateral follicle cell clones do not result in defects in the dorso-ventral patterning of the embryo, while ventral follicle cell clones produce local dorsalisation in the embryo. cic1/cicfet-U6 females produce embryos with a tor gain-of-function phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

cicfet-U6/cicfet-T6 has egg phenotype, suppressible by mirr6D1

cicfet-U6/cicfet-E11 has egg phenotype, suppressible by mirr6D1

cicfet-U6/cicBA53 has egg phenotype, suppressible by mirr6D1

NOT suppressed by
Statement
Reference

cicfet-U6/cicfet-T6 has egg phenotype, non-suppressible by grk3/grk3/grk2B6

cicfet-U6/cicfet-T6 has dorsal appendage phenotype, non-suppressible by rho6

cicfet-U6/cicfet-T6 has egg phenotype, non-suppressible by rho6

Additional Comments
Genetic Interactions
Statement
Reference

Approximately 0.5% of cicfet-T6/mirr6D1 cicfet-U6 females exhibit a weak eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally with a trace of ventral collar material. Approximately 42.6% of cicfet-U6/mirr6D1 cicfet-T6 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. Approximately 42.1% of cicfet-U6/mirr6D1 cicfet-T6 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. None of the cicfet-U6/mirr6D1 cicfet-T6 females exhibit a severe eggshell phenotype (compared to 1.7% in the heterozygote). There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicfet-T6/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicfet-T6/cicfet-U6 phenotype.

Approximately 34.4% of cicfet-E11/mirr6D1 cicfet-U6 females exhibit a weak eggshell phenotype. Approximately 37.4% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a weak-moderate eggshell phenotype. Approximately 24.5% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a moderate eggshell phenotype. Approximately 37.9% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicfet-E11/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicfet-E11/cicfet-U6 phenotype.

Approximately 44.6% of cicfet-U6/mirr6D1 cicfet-T6 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 37.9% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material.

Approximately 0.5% of cicfet-U6/mirr6D1 cicBA53 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. Approximately 32.6% of cicfet-U6/mirr6D1 cicBA53 females exhibit a severe eggshell phenotype (compared to 85.9 in the heterozygote). The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicBA53/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicBA53/cicfet-U6 phenotype.

Stage 12 grk3/grk2B6 ; cicfet-U6/cicfet-T6 double mutant egg chambers lack dorsal appendages (characteristic of loss of grk), but retain a pronounced collar of appendage material, indicating that loss of grk cannot suppress the cic phenotype.

rho6 mutant clones have no effect on the ectopic appendage-producing fates in cicfet-U6/cicfet-T6 mutant egg chambers, indicating that loss of rho function cannot suppress the cic phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a mutant that shows extra wing veins or blisters over cicfet-E11.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (10)