FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\grk12xTag:MS2
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General Information
Symbol
Dmel\grk12xTag:MS2
Species
D. melanogaster
Name
FlyBase ID
FBal0296112
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
grk-(MS2)12
Key Links
Nature of the Allele
Progenitor genotype
Carried in construct
Cytology
Description

5050bp genomic fragment containing the entire grk locus. Twelve copies of Tag:MS2, the stem-loop binding site for Tag:MCP, have been introduced into the grk 3'UTR sequence at position 1603 in the mRNA sequence (343bp after the stop codon). The version of Tag:MS2 used is a mutated version that results in very strong binding affinity.

Allele components
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
oocyte | anterior

Comment: transient cortical ring

Additional Information
Statement
Reference

Live imaging shows reporter expression lining the posterior of the oocyte in early stages of oogenesis (stages S4-7). During stage 7, the oocyte nucleus shifts and reporter accumulates around the anterior cortex as well as lining the posterior. At stage S8, the oocyte nucleus moves to one side. Labelling at the posterior and lateral sides of the oocyte decreases and is observed instead is seen to line the lateral wall posterior to the nucleus. The dorsal-anterior cap above the nucleus becomes increasingly prominent. As stage S8 progresses, the anterior ring becomes more prominent and little staining is seen posteriorly. In stages S9-10, staining becomes more prominent in the dorsal-anterior cap above the nucleus.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
grk12x.T:MS2\MCP.BS
grk12x.Tag:BS(MS2-CP)
grk12xTag:MS2
Name Synonyms
Secondary FlyBase IDs
    References (6)